Anti-CD14 antibody (ab45870)
Key features and details
- Goat polyclonal to CD14
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-CD14 antibody
See all CD14 primary antibodies -
Description
Goat polyclonal to CD14 -
Host species
Goat -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide within Human CD14 aa 50-150 (internal sequence). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
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Positive control
- WB: Human blood mononucleocyte lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituent: 0.5% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-CD14 antibody (ab45870)All lanes : Anti-CD14 antibody (ab45870) at 1 µg/ml
Lane 1 : Human Peripheral Blood Mononucleocyte lysate
Lane 2 : Human Peripheral Blood Mononucleocyte lysate with Immunizing peptide
Lysates/proteins at 35 µg per lane.
Predicted band size: 40 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?
Additional bands at: 15 kDa. We are unsure as to the identity of these extra bands.The observed molecular weight corresponds to the glycosylated form.
Primary incubation 1 hour at room temperature. Detected by chemiluminescence.
