Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CD14 antibody [EPR3653] (ab133335)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3653] to CD14
  • Suitable for: WB, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-CD14 antibody [EPR3653]
    See all CD14 primary antibodies

  • Description

    Rabbit monoclonal [EPR3653] to CD14

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: human colon, placenta and tonsil tissue; WB: Human tonsil tissue lysate, SW480 and PBMCl lysates.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD14 antibody [EPR3653] (ab133335)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD14 antibody [EPR3653] (ab133335)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon tissue sections labeling CD14 with purified ab133335 at 1/2000 dilution (0.04 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

  • Western blot - Anti-CD14 antibody [EPR3653] (ab133335)
    Western blot - Anti-CD14 antibody [EPR3653] (ab133335)
    Anti-CD14 antibody [EPR3653] (ab133335) at 1/1000 dilution (purified) + His-Tagged Human CD14 (aa20 to 345) recombinant protein at 0.015 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 40 kDa
    Observed band size: 43 kDa
    why is the actual band size different from the predicted?


    Exposure time: 1 second


    Blocking and diluting buffer: 5% NFDM/TBST

  • Western blot - Anti-CD14 antibody [EPR3653] (ab133335)
    Western blot - Anti-CD14 antibody [EPR3653] (ab133335)
    All lanes : Anti-CD14 antibody [EPR3653] (ab133335) at 1/1000 dilution

    Lane 1 : Human tonsil lysates prepared in RIPA lysis method
    Lane 2 : Human tonsil lysates prepared in 1%SDS Hot lysis method

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 40 kDa


    Exposure time: 45 seconds


    Blocking/Diluting buffer and concentration: 5% NFDM/TBST

  • Western blot - Anti-CD14 antibody [EPR3653] (ab133335)
    Western blot - Anti-CD14 antibody [EPR3653] (ab133335)
    All lanes : Anti-CD14 antibody [EPR3653] (ab133335) at 1/1000 dilution (purified)

    Lane 1 : Human tonsil tissue lysate prepared in 1% SDS Hot lysis method
    Lane 2 : HeLa (Human cervix adenocarcinoma) whole cell lysate
    Lane 3 : U-937 (Human histiocytic lymphoma) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Lane 1 : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
    Lanes 2-3 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 40 kDa
    Observed band size: 53 kDa why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking and diluting buffer: 5% NFDM/TBST

    The expression level in HeLa and U-937 are low (PMID: 9886426 and 15730927)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD14 antibody [EPR3653] (ab133335)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD14 antibody [EPR3653] (ab133335)

    Immunohistochemical analysis of Formalin-fixed, paraffin- embedded human tonsil tissue labelling CD14 with ab133335 (unpurified) at 1/500 dilution. No blocking step performed. Anti-Rabbit HRP polymer was used as the secondary detection system. Heat-mediated antigen retrieval was performed using EDTA based pH 9.0 buffer.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD14 antibody [EPR3653] (ab133335)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD14 antibody [EPR3653] (ab133335)

    Immunohistochemical analysis of Formalin-fixed, paraffin- embedded Human tonsil tissue labelling CD14 with ab133335 (unpurified) at 1/500 dilution. Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD14 antibody [EPR3653] (ab133335)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD14 antibody [EPR3653] (ab133335)

    Immunohistochemical analysis of Formalin-fixed, paraffin- embedded Human placenta tissue labelling CD14 with ab133335 (unpurified) at 1/500 dilution. Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

  • Western blot - Anti-CD14 antibody [EPR3653] (ab133335)
    Western blot - Anti-CD14 antibody [EPR3653] (ab133335)
    All lanes : Anti-CD14 antibody [EPR3653] (ab133335) at 1/1000 dilution (unpurified)

    Lane 1 : PBMC cell lysate prepared in 1% SDS Hot lysis method
    Lane 2 : Human tonsil tissue lysate prepared in 1%SDS Hot lysis method
    Lane 3 : SW480 cell lysate prepared in 1%SDS Hot lysis method

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 40 kDa
    Observed band size: 53 kDa why is the actual band size different from the predicted?

  • OI-RD Scanning - Anti-CD14 antibody [EPR3653] (ab133335)
    OI-RD Scanning - Anti-CD14 antibody [EPR3653] (ab133335)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-CD14 antibody [EPR3653] (ab133335)
    Anti-CD14 antibody [EPR3653] (ab133335)

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