Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-IRG1 antibody [EPR22066] - BSA and Azide free (ab238169)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22066] to IRG1 - BSA and Azide free
  • Suitable for: WB, IP
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-IRG1 antibody [EPR22066] - BSA and Azide free
    See all IRG1 primary antibodies

  • Description

    Rabbit monoclonal [EPR22066] to IRG1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Mouse
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: RAW 664.7 (treated with 100 ng/ml lipopolysaccharide (LPS) for 4 hours) and THP-1 (100 ng/ml phorbol-12-myristate-13-acetate (PMA) for 72 hours, then with 1 µg/ml LPS after 52 hours) whole cell lysates.

  • General notes

    Ab238169 is the carrier-free version of ab222411. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab238169 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunoprecipitation - Anti-IRG1 antibody [EPR22066] - BSA and Azide free (ab238169)
    Immunoprecipitation - Anti-IRG1 antibody [EPR22066] - BSA and Azide free (ab238169)

    IRG1 was immunoprecipitated from 0.35 mg of THP-1 (human monocytic leukemia cell line)(treated with 100 ng/ml phorbol-12-myristate-13-acetate (PMA) for 72 hours, then with 1 μg/ml LPS added for 16 hours) whole cell lysate with ab222411 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab222411 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1/5000 dilution.

    Lane 1: THP-1 (treated with PMA/LPS) whole cell lysate, 10 μg (Input).
    Lane 2: ab222411 IP in THP-1 (treated with PMA/LPS) whole cell lysate.
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab222411 in THP-1 (treated with PMA/LPS) whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 8 seconds.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab222411).

  • Western blot - Anti-IRG1 antibody [EPR22066] - BSA and Azide free (ab238169)
    Western blot - Anti-IRG1 antibody [EPR22066] - BSA and Azide free (ab238169)
    All lanes : Anti-IRG1 antibody [EPR22066] (ab222411) at 1/1000 dilution

    Lane 1 : Untreated RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
    Lane 2 : RAW 264.7 (treated with 100 ng/ml lipopolysaccharide (LPS) for 4 hours) whole cell lysate
    Lane 3 : Untreated THP-1 (human monocytic leukemia cell line) whole cell lysate
    Lane 4 : THP-1 (treated with 100 ng/ml phorbol-12-myristate-13-acetate (PMA) for 72 hours, then with 1 µg/ml LPS after 52 hours) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 53 kDa
    Observed band size: 50 kDa
    why is the actual band size different from the predicted?



    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure times.

    Lanes 1 & 2: 3 minutes.

    Lanes 3 & 4: 8 seconds.

    The molecular mass observed is consistent with what has been described in the literature (PMID: 23610393 and PMID: 28699638).
    The protein is up-regulated after lipopolysaccharide (LPS) stimulation (PMID: 25640654 and PMID: 26679997).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab222411).

     

  • Anti-IRG1 antibody [EPR22066] - BSA and Azide free (ab238169)
    Anti-IRG1 antibody [EPR22066] - BSA and Azide free (ab238169)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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