Anti-ERCC1 antibody (ab227286)
Key features and details
- Rabbit polyclonal to ERCC1
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-ERCC1 antibody
See all ERCC1 primary antibodies -
Description
Rabbit polyclonal to ERCC1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Human
-
Immunogen
Recombinant fragment within Human ERCC1 (internal sequence). The exact sequence is proprietary.
Database link: P07992 -
Positive control
- WB: HeLa cell extract. ICC/IF: A549 cells.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-ERCC1 antibody (ab227286)All lanes : Anti-ERCC1 antibody (ab227286) at 1/1000 dilution
Lane 1 : WT HeLa cell extract
Lane 2 : ERCC1 Knockout HeLa cell extracts
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
-
Western blot - Anti-ERCC1 antibody (ab227286)All lanes : Anti-ERCC1 antibody (ab227286) at 1/1000 dilution
Lane 1 : WT HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : ERCC1 knockout HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG10% SDS-PAGE gel.
-
Immunocytochemistry/ Immunofluorescence - Anti-ERCC1 antibody (ab227286)A549 (human lung carcinoma cell line) cells stained for ERCC1 (green) using ab227286 at 1/1000 dilution in ICC/IF. Cells were fixed in 4% paraformaldehyde at RT for 15 minutes. Nuclear counterstain: Hoechst 33342 (blue).
