Antibodies, Proteins, Kits and Reagents for Life Science

321 638 ₸ Product size

100 µl 321 638 ₸

Order now and get it on Tuesday March 02, 2021

Anti-ERCC1 antibody [EPR7277] (ab129267)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR7277] to ERCC1
Suitable for: WB, IHC-P, Flow Cyt, ICC/IF
Reacts with: Human

Product name

Anti-ERCC1 antibody [EPR7277]
See all ERCC1 primary antibodies
Description

Rabbit monoclonal [EPR7277] to ERCC1
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide within Human ERCC1 aa 1-200. The exact sequence is proprietary.
Database link: P07992
Positive control
- Human lung carcinoma tissue; HeLa, HepG2 and SKBR-3 cell lysates. HepG2 cells.
General notes
Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR7277
Isotype

IgG
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERCC1 antibody [EPR7277] (ab129267)

Formalin-fixed, paraffin-embedded human lung carcinoma tissue stained for ERCC1 with ab129267 (1/100 dilution) in immunohistochemical analysis.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-ERCC1 antibody [EPR7277] (ab129267)

ab129267, at 1/100 dilution, staining ERCC1 in HepG2 cells by Immunofluorescence.
Western blot - Anti-ERCC1 antibody [EPR7277] (ab129267)

All lanes : Anti-ERCC1 antibody [EPR7277] (ab129267) at 1/1000 dilution

Lane 1 : HeLa cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : SKBR-3 cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-rabbit HRP

Predicted band size: 33 kDa
Observed band size: 36 kDa
why is the actual band size different from the predicted?
Flow Cytometry - Anti-ERCC1 antibody [EPR7277] (ab129267)

Flow Cytometry analysis of HepG2 (human hepatocellular carcinoma) cells labeling ERCC1 with purified ab129267 at 1/80 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor^® 488)(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.
Anti-ERCC1 antibody [EPR7277] (ab129267)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com