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Anti-ERCC1 antibody [1E5B3] (ab204988)

Anti-ERCC1 antibody [1E5B3] (ab204988)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Mouse monoclonal [1E5B3] to ERCC1
  • Suitable for: Flow Cyt, WB, ICC/IF
  • Reacts with: Human
  • Isotype: IgG1

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Overview

  • Product name

    Anti-ERCC1 antibody [1E5B3]
    See all ERCC1 primary antibodies
  • Description

    Mouse monoclonal [1E5B3] to ERCC1
  • Host species

    Mouse
  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Recombinant fragment corresponding to Human ERCC1 aa 150 to the C-terminus. (Purified from E.coli).
    Database link: P07992

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • Human ERCC1 (aa151-297) recombinant protein; ERCC1 (aa151-297)-hIgGFc transfected HEK293 cell lysate; HeLa and A549 cells.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.05% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    Purified from tissue culture supernatant.
  • Clonality

    Monoclonal
  • Clone number

    1E5B3
  • Isotype

    IgG1
  • Research areas

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • DNA Damage & Repair
    • Nucl. Excision Repair
    • Cancer
    • Tumor biomarkers
    • Other

Images

  • Western blot - Anti-ERCC1 antibody [1E5B3] (ab204988)
    Western blot - Anti-ERCC1 antibody [1E5B3] (ab204988)
    Anti-ERCC1 antibody [1E5B3] (ab204988) at 1/500 dilution + Human ERCC1 (aa151-297) recombinant protein

    Predicted band size: 33 kDa



    Expected MWt is 42.5 kDa.

  • Western blot - Anti-ERCC1 antibody [1E5B3] (ab204988)
    Western blot - Anti-ERCC1 antibody [1E5B3] (ab204988)
    All lanes : Anti-ERCC1 antibody [1E5B3] (ab204988) at 1/500 dilution

    Lane 1 : HEK293 cell lysate
    Lane 2 : ERCC1 (aa151-297)-hIgGFc transfected HEK293 cell lysate

    Predicted band size: 33 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-ERCC1 antibody [1E5B3] (ab204988)
    Immunocytochemistry/ Immunofluorescence - Anti-ERCC1 antibody [1E5B3] (ab204988)

    Immunofluorescent analysis of HeLa cells labeling ERCC1 with ab204988 at 1/200 dilution (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.

  • Flow Cytometry - Anti-ERCC1 antibody [1E5B3] (ab204988)
    Flow Cytometry - Anti-ERCC1 antibody [1E5B3] (ab204988)

    Flow cytometric analysis of A549 cells labeling ERCC1 with ab204988 at 1/200 dilution (green), compared to a negative control (red).

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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