Anti-ERCC1 antibody [1E5B3] (ab204988)
Key features and details
- Mouse monoclonal [1E5B3] to ERCC1
- Suitable for: Flow Cyt, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-ERCC1 antibody [1E5B3]
See all ERCC1 primary antibodies -
Description
Mouse monoclonal [1E5B3] to ERCC1 -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC/IF HumanWB Human -
Immunogen
Recombinant fragment corresponding to Human ERCC1 aa 150 to the C-terminus. (Purified from E.coli).
Database link: P07992 -
Positive control
- Human ERCC1 (aa151-297) recombinant protein; ERCC1 (aa151-297)-hIgGFc transfected HEK293 cell lysate; HeLa and A549 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purified from tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
1E5B3 -
Isotype
IgG1 -
Research areas
Images
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Anti-ERCC1 antibody [1E5B3] (ab204988) at 1/500 dilution + Human ERCC1 (aa151-297) recombinant protein
Predicted band size: 33 kDaExpected MWt is 42.5 kDa.
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All lanes : Anti-ERCC1 antibody [1E5B3] (ab204988) at 1/500 dilution
Lane 1 : HEK293 cell lysate
Lane 2 : ERCC1 (aa151-297)-hIgGFc transfected HEK293 cell lysate
Predicted band size: 33 kDa
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Immunofluorescent analysis of HeLa cells labeling ERCC1 with ab204988 at 1/200 dilution (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
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Flow cytometric analysis of A549 cells labeling ERCC1 with ab204988 at 1/200 dilution (green), compared to a negative control (red).