Anti-GABARAPL1 antibody (ab229729)
Key features and details
- Rabbit polyclonal to GABARAPL1
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-GABARAPL1 antibody
See all GABARAPL1 primary antibodies -
Description
Rabbit polyclonal to GABARAPL1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Guinea pig, Cow, Pig, Xenopus laevis, Rhesus monkey
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Immunogen
Recombinant full length protein corresponding to Human GABARAPL1.
Database link: Q9H0R8 -
Positive control
- WB: HEK-293T and HeLa whole cell lysates. ICC/IF: HeLa cells (+/- treatment with Chloroquine).
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-GABARAPL1 antibody (ab229729)All lanes : Anti-GABARAPL1 antibody (ab229729) at 1/500 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 14 kDa15% SDS-PAGE gel.
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Western blot - Anti-GABARAPL1 antibody (ab229729)All lanes : Anti-GABARAPL1 antibody (ab229729) at 1/1000 dilution
Lane 1 : Non-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : GABARAPL1-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 14 kDa15% SDS-PAGE gel.
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Immunocytochemistry/ Immunofluorescence - Anti-GABARAPL1 antibody (ab229729)4% paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells untreated (left panel) and treated with 50 μM Chloroquine for 24 hours (right panel) stained for GABARAPL1 (green) using ab229729 at 1/1000 dilution in ICC/IF.
Red: Phalloidin, a F-actin marker.
