Anti-GABARAPL1 antibody - N-terminal (ab229558)
Key features and details
- Rabbit polyclonal to GABARAPL1 - N-terminal
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-GABARAPL1 antibody - N-terminal
See all GABARAPL1 primary antibodies -
Description
Rabbit polyclonal to GABARAPL1 - N-terminal -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Guinea pig, Cow, Pig, Xenopus laevis, Rhesus monkey
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Immunogen
Synthetic peptide within Human GABARAPL1 (N terminal). The exact sequence is proprietary. Conjugated to a protein carrier.
Database link: Q9H0R8 -
Positive control
- ICC/IF: Chloroquine treated HeLa cells. WB: U-87 MG and SK-N-SH whole cell extracts; mouse and rat brain tissue extracts.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-GABARAPL1 antibody - N-terminal (ab229558)
HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for GABARAPL1 (green) using ab229558 (1/500 dilution) in ICC/IF. Cells were fixed in 4% paraformaldehyde at RT for 15 minutes. Counterstain: Hoechst 33342 (blue) and phalloidin, a cytoskeleton marker (red)
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Western blot - Anti-GABARAPL1 antibody - N-terminal (ab229558)All lanes : Anti-GABARAPL1 antibody - N-terminal (ab229558) at 1/1000 dilution
Lane 1 : U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) whole cell extracts at 60 µg
Lane 2 : SK-N-SH (Human neuroblastoma cell line) whole cell extracts at 60 µg
Lane 3 : Mouse brain tissue extracts at 15 µg
Lane 4 : Rat brain tissue extracts at 15 µg
Developed using the ECL technique.
Predicted band size: 14 kDa15% SDS-PAGE
