Anti-Nsr1p antibody [31C4] (ab4642)
![Anti-Nsr1p antibody [31C4] (ab4642)](https://www.abcam.com/ps/products/4/ab4642/Images/Nsr1p-Primary-antibodies-ab4642-5.jpg "Anti-Nsr1p antibody [31C4] (ab4642)")
Key features and details
- Mouse monoclonal [31C4] to Nsr1p
- Suitable for: ICC/IF, WB
- Reacts with: Saccharomyces cerevisiae
- Isotype: IgG
Overview
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Product name
Anti-Nsr1p antibody [31C4] -
Description
Mouse monoclonal [31C4] to Nsr1p -
Host species
Mouse -
Specificity
This antibody was isolated during a screen for mAbs against yeast nucleolar proteins. Specificity was determined by screening a yeast genomic library in lambda gt11. Two individually isolated, different positive clones were found. Sequencing revealed that in both clones beta-galactosidase was fused at the EcoRI site to the amino acid sequence beginning with …EFEH190 in Nsr1p. Thus, it is likely to react with an epitope(s) in the C-terminal 55% of Nsr1p. -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Saccharomyces cerevisiae -
Immunogen
This was raised against a yeast nucleolar prep, and screened by immunocytochemistry and western blotting.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.065% Sodium azide
Constituent: Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Purification notes
Sterile filtered. -
Clonality
Monoclonal -
Clone number
31C4 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Nsr1p antibody [31C4] (ab4642) Image courtesy of an anonymous Abreview.All lanes : Anti-Nsr1p antibody [31C4] (ab4642) at 1/10000 dilution
Lane 1 : Saccharomyces cerevisae DDY1810 (WT) whole cell lysate
Lane 2 : Saccharomyces cerevisae DDY1810 (nsr1 knockout) whole cell lysate
Secondary
All lanes : Rabbit anti-mouse IgG conjugated to peroxidase at 1/30000 dilution
Performed under reducing conditions.
Predicted band size: 45 kDa
Bottom panel: loading control performed using Saccharomyces cerevisae anti-HSP60 (1:1000) -
![Immunocytochemistry/ Immunofluorescence - Anti-Nsr1p antibody [31C4] (ab4642)](https://www.abcam.com/ps/products/4/ab4642/Images/Nsr1p-Primary-antibodies-ab4642-7.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Nsr1p antibody [31C4] (ab4642) Image courtesy of an anonymous Abreview.ab4642 (1/5000) detecting Nsr1p inDDY1810 (red). Cells were fixed in paraformaldehyde, permeabilized with methanol/acetone and counterstained with DAPI in order to highlight the nucleus (blue).
Right hand panel: negative control performed using Saccharomyces cerevisae DDY1810 nsr1p knockout.
For further experimental details please refer to Abreview.