Anti-BARD1 antibody [2059C4a] (ab50984)
![Anti-BARD1 antibody [2059C4a] (ab50984)](https://www.abcam.com/ps/products/50/ab50984/Images/ab50984-62793-anti-bard1-antibody-2059c4a-western-blot.jpg "Anti-BARD1 antibody [2059C4a] (ab50984)")
Key features and details
- Mouse monoclonal [2059C4a] to BARD1
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-BARD1 antibody [2059C4a]
See all BARD1 primary antibodies -
Description
Mouse monoclonal [2059C4a] to BARD1 -
Host species
Mouse -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment (Human)
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Positive control
- HEK293 whole cell lysate, RIPA lysate of HeLa cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 1% BSA, PBS -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Protein G purified from culture of supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (~95%) fetal bovine serum. Filtered through a 0.22 µm membrane. -
Clonality
Monoclonal -
Clone number
2059C4a -
Isotype
IgG1 -
Research areas
Images
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Western blot - Anti-BARD1 antibody [2059C4a] (ab50984)Anti-BARD1 antibody [2059C4a] (ab50984) at 1/50 dilution + HEK293 whole cell lysate at 50 µg
Secondary
Anti mouse IgG antibody at 1/2500 dilution
Predicted band size: 86 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?
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![Immunoprecipitation - Anti-BARD1 antibody [2059C4a] (ab50984)](https://www.abcam.com/ps/products/50/ab50984/Images/ab50984-62792-anti-bard1-antibody-2059c4a-immunoprecipitation.jpg)
Immunoprecipitation - Anti-BARD1 antibody [2059C4a] (ab50984)Ab50984 at 1/50 dilution precipitating RIPA lysate of HeLa cells at 500µg; visualised with anti mouse IgG antibody at 1/2500 diltuion.
