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Anti-BARD1 antibody (ab226854)

Price and availability

278 083 ₸

Availability

Order now and get it on Wednesday February 24, 2021

Anti-BARD1 antibody (ab226854)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to BARD1
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-BARD1 antibody
    See all BARD1 primary antibodies
  • Description

    Rabbit polyclonal to BARD1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human BARD1 (internal sequence). The exact sequence is proprietary.
    Database link: Q99728

  • Positive control

    • WB: A431 whole cell and nuclear extracts. IHC-P: Mouse and rat brain tissues. ICC/IF: A431 cells.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.025% Proclin 300
    Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • DNA Damage & Repair
    • DNA Damage Response
    • DNA Damage Recognition
    • Cell Biology
    • Proteolysis / Ubiquitin
    • Proteasome / Ubiquitin
    • Ubiquitin E3 Enzymes
    • RING Finger E3 Ligase
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Cancer susceptibility
    • Tumor Suppressors
    • Epigenetics and Nuclear Signaling
    • Cell cycle
    • Apoptosis
    • Nuclear
    • Cancer
    • Oncoproteins/suppressors
    • Tumor suppressors
    • Other
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Apoptosis
    • Cancer
    • Cell Death
    • Apoptosis
    • Metabolism

Images

  • Western blot - Anti-BARD1 antibody (ab226854)
    Western blot - Anti-BARD1 antibody (ab226854)
    All lanes : Anti-BARD1 antibody (ab226854) at 1/1000 dilution

    Lane 1 : A431 (human epidermoid carcinoma cell line) whole cell extract
    Lane 2 : A431 nuclear extract

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 87 kDa



    7.5% SDS-PAGE gel.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BARD1 antibody (ab226854)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BARD1 antibody (ab226854)

    Paraffin-embedded mouse brain tissue stained for BARD1 with ab226854 at 1/400 dilution in immunohistochemical analysis

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BARD1 antibody (ab226854)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BARD1 antibody (ab226854)

    Paraffin-embedded rat brain tissue stained for BARD1 with ab226854 at 1/400 dilution in immunohistochemical analysis

  • Immunocytochemistry/ Immunofluorescence - Anti-BARD1 antibody (ab226854)
    Immunocytochemistry/ Immunofluorescence - Anti-BARD1 antibody (ab226854)

    4% paraformaldehyde-fixed A431 (human epidermoid carcinoma cell line) cells stained for BARD1 (green) using ab226854 at 1/500 dilution in ICC/IF.

    Blue: Hoechst 33342 staining.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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