Anti-CHM antibody [EPR7142] (ab134964)
![Anti-CHM antibody [EPR7142] (ab134964)](https://www.abcam.com/ps/products/134/ab134964/Images/ab134964-149202-anti-chm-antibody-epr7142-western-blot.jpg "Anti-CHM antibody [EPR7142] (ab134964)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7142] to CHM
- Suitable for: WB, ICC/IF
- Reacts with: Human
Overview
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Product name
Anti-CHM antibody [EPR7142]
See all CHM primary antibodies -
Description
Rabbit monoclonal [EPR7142] to CHM -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Human
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Immunogen
Synthetic peptide corresponding to residues in Human CHM (UniProt ID: P24386).
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Positive control
- HeLa, Y79, A431, and 293T cell lysates. A431 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR7142 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-CHM antibody [EPR7142] (ab134964)All lanes : Anti-CHM antibody [EPR7142] (ab134964) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : Y79 cell lysate
Lane 3 : A431 cell lysate
Lane 4 : 293T cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 73 kDa
Observed band size: 95 kDa why is the actual band size different from the predicted?
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![Immunocytochemistry/ Immunofluorescence - Anti-CHM antibody [EPR7142] (ab134964)](https://www.abcam.com/ps/products/134/ab134964/Images/ab134964-149201-anti-chm-antibody-epr7142-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-CHM antibody [EPR7142] (ab134964)Immunofluorescence analysis of CHM in A431 cells, using ab134964 at a dilution of 1/250. -
![Anti-CHM antibody [EPR7142] (ab134964)](https://www.abcam.com/ps/products/134/ab134964/Images/ab134964-3-benefits-of-recombinant-antibodies.png)
Anti-CHM antibody [EPR7142] (ab134964)
