Anti-BMAL1 antibody (ab3350)
Key features and details
- Rabbit polyclonal to BMAL1
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-BMAL1 antibody
See all BMAL1 primary antibodies -
Description
Rabbit polyclonal to BMAL1 -
Host species
Rabbit -
Specificity
Detects BMAL 1 / aryl hydrocarbon nuclear translocator 3 (ARNT 3) from hamster tissues as well as recombinant human BMAL 1. -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Horse, Chicken
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Immunogen
Synthetic peptide corresponding to Mouse BMAL1 aa 582-594.
Sequence:DMIDNDQGSSSPS
(Peptide available asab4959)
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-BMAL1 antibody (ab3350)All lanes : Anti-BMAL1 antibody (ab3350) at 1/500 dilution
Lane 1 : U251 cell lysate
Lane 2 : U87-MG cell lysate
Lane 3 : NIH-3T3 cell lysate
Lysates/proteins at 25 µg per lane.
Predicted band size: 69.4 kDa
Observed band size: 69 kDa why is the actual band size different from the predicted?
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Immunocytochemistry/ Immunofluorescence - Anti-BMAL1 antibody (ab3350)ICC/IF image of ab3350 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab3350, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Western blot - Anti-BMAL1 antibody (ab3350)Anti-BMAL1 antibody (ab3350) at 1 µg/ml + HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Predicted band size: 69.4 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?
Additional bands at: 51 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 3 minutes
The 75 kDa band observed is comparable to the molecular weight seen with other commercially available antibodies to BMAL1.
