Anti-BMAL1 antibody (ab228594)
Key features and details
- Rabbit polyclonal to BMAL1
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-BMAL1 antibody
See all BMAL1 primary antibodies -
Description
Rabbit polyclonal to BMAL1 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Sheep, Chicken, Cow, Pig, Xenopus laevis, Rhesus monkey -
Immunogen
Recombinant fragment within Human BMAL1 (internal sequence). The exact sequence is proprietary.
Database link: O00327 -
Positive control
- WB: BMAL1 transfected HEK-293T whole cell lysate; Mouse brain lysate. ICC/IF: A-375 cells.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Anti-BMAL1 antibody (ab228594) at 1/1000 dilution + Mouse brain lysate at 50 µg
Predicted band size: 69 kDa7.5% SDS-PAGE gel.
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All lanes : Anti-BMAL1 antibody (ab228594) at 1/500 dilution
Lane 1 : Non-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with BMAL1, whole cell lysate
Predicted band size: 69 kDa7.5% SDS-PAGE gel.
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4% paraformaldehyde-fixed A-375 (human malignant melanoma cell line) cells stained for BMAL1 (green) using ab228594 at 1/500 dilution in ICC/IF.
Nuclear counter stain: Hoechst 33342 (blue).