Mouse TRAIL ELISA Kit (ab253210)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 33.79 pg/ml
- Range: 78.125 pg/ml - 5000 pg/ml
- Sample type: Cell culture media, Cit plasma, EDTA Plasma, Serum, Tissue Extracts
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse TRAIL ELISA Kit
See all TRAIL kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 5 4.8% Inter-assay Sample n Mean SD CV% Serum 3 3.2% -
Sample type
Serum, Tissue Extracts, Cell culture media, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
33.79 pg/ml -
Range
78.125 pg/ml - 5000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 87 80% - 94% Tissue Extracts 110 105% - 116% Cell culture media 114 111% - 119% EDTA Plasma 87 80% - 101% Cit plasma 86 84% - 88% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Mouse -
Product overview
Mouse TRAIL ELISA Kit (ab253210) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of TRAIL protein in cit plasma, edta plasma, serum, tissue extracts, and cell culture media. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse TRAIL with 33.79 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
TRAIL (Tnfsf10) is a type II transmembrane protein that is a cytokine member of the TNFL superfamily. TRAIL activation leads to NFkappaB induction of apoptosis, making it an attractive target as a drug target for cancer. Chemical induction of TRAIL expression in mice was able to induce a partial reduction in the number of cancerous cells.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Mouse TRAIL Capture Antibody 1 x 600µl 10X Mouse TRAIL Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 4BI 1 x 6ml Mouse TRAIL Lyophilized recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent 50BP 1 x 20ml Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Cytokine that binds to TNFRSF10A/TRAILR1, TNFRSF10B/TRAILR2, TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and possibly also to TNFRSF11B/OPG. Induces apoptosis. Its activity may be modulated by binding to the decoy receptors TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and TNFRSF11B/OPG that cannot induce apoptosis. -
Tissue specificity
Widespread; most predominant in spleen, lung and prostate. -
Sequence similarities
Belongs to the tumor necrosis factor family. -
Cellular localization
Membrane. - Information by UniProt
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Alternative names
- Apo 2 ligand
- APO 2L
- Apo-2 ligand
see all -
Database links
- Entrez Gene: 22035 Mouse
- SwissProt: P50592 Mouse
- Unigene: 1062 Mouse
Images
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Other - Mouse TRAIL ELISA Kit (ab253210)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of mouse TRAIL standard curve in Sample Diluent 50BP.The TRAIL standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Example of mouse TRAIL standard curve in 1X Cell Extraction Buffer PTR.The TRAIL standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native TRAIL in mouse kidney and thymus homogenate extract samples based on a 1,000 µg/mL extract load.The concentrations of TRAIL were measured in duplicate and interpolated from the TRAIL standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean TRAIL concentration was determined to be 595.83 pg/mL in kidney homogenate extract and 268.83 pg/mL in thymus homogenate extract.
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Sandwich ELISA - Mouse TRAIL ELISA Kit (ab253210)To learn more about the advantages of recombinant antibodies see here.
