Anti-LIM Kinase 1 antibody [1B11] (ab243137)
![Anti-LIM Kinase 1 antibody [1B11] (ab243137)](https://www.abcam.com/ps/products/243/ab243137/Images/ab243137-391687-anti-lim-ki-se-1-antibody-1b11-western-blot-human-brain-tissue-lysate20-ug.jpg "Anti-LIM Kinase 1 antibody [1B11] (ab243137)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rat monoclonal [1B11] to LIM Kinase 1
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-LIM Kinase 1 antibody [1B11]
See all LIM Kinase 1 primary antibodies -
Description
Rat monoclonal [1B11] to LIM Kinase 1 -
Host species
Rat -
Tested Applications & Species
See all applications and species dataApplication Species WB MouseRatHuman
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Immunogen
Recombinant fragment within Mouse LIM Kinase 1. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
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Positive control
- WB: Human, mouse and rat brain tissue lysate.
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 30% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Ion Exchange Chromatography -
Clonality
Monoclonal -
Clone number
1B11 -
Isotype
IgG2a -
Research areas
Images
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Western blot - Anti-LIM Kinase 1 antibody [1B11] (ab243137)All lanes : Anti-LIM Kinase 1 antibody [1B11] (ab243137) at 1/1000 dilution
Lane 1 : Human brain tissue lysate
Lane 2 : Rat brain tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rat IgG (H+L), HRP) (ab205720) at 1/10000 dilution
Predicted band size: 73 kDa
Observed band size: 73 kDaBlocking and diluting buffer and concentration: 5% NFDM/TBST.
Exposure time: Lane 1 : 180 seconds; Lane 2 : 10 seconds.
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![Western blot - Anti-LIM Kinase 1 antibody [1B11] (ab243137)](https://www.abcam.com/ps/products/243/ab243137/Images/ab243137-391688-anti-lim-ki-se-1-antibody-1b11-western-blot-mosue-brain-tissue-lysate20-ug.jpg)
Western blot - Anti-LIM Kinase 1 antibody [1B11] (ab243137)All lanes : Anti-LIM Kinase 1 antibody [1B11] (ab243137) at 1/1000 dilution
Lane 1 : Mosue brain tissue lysate
Lane 2 : Mouse liver tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rat IgG (H+L), HRP) (ab205720) at 1/10000 dilution
Predicted band size: 73 kDa
Observed band size: 73 kDaBlocking and diluting buffer and concentration: 5% NFDM/TBST.
Negative control: Mouse liver (PMID: 8537403).
Exposure time: 81 seconds.
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![Anti-LIM Kinase 1 antibody [1B11] (ab243137)](https://www.abcam.com/ps/products/243/ab243137/Images/ab243137-3-benefits-of-recombinant-antibodies.png)
Anti-LIM Kinase 1 antibody [1B11] (ab243137)