Anti-SHP1 (phospho Y564) antibody (ab79294)
Key features and details
- Rabbit polyclonal to SHP1 (phospho Y564)
- Suitable for: WB
- Reacts with: African green monkey
- Isotype: IgG
Overview
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Product name
Anti-SHP1 (phospho Y564) antibody
See all SHP1 primary antibodies -
Description
Rabbit polyclonal to SHP1 (phospho Y564) -
Host species
Rabbit -
Specificity
ab79294 detects endogenous levels of SHP1 only when phosphorylated at tyrosine 564. -
Tested Applications & Species
See all applications and species dataApplication Species WB African green monkey -
Immunogen
Syntheticphosphopeptide derived from human SHP1 around the phosphorylation site of tyrosine 564 (DVYPEN).
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Positive control
- Extracts from COS7 cells, treated with EGF (200ng/ml, 30mins)
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab79294 is purified from rabbit antiserum by chromatography using an epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-SHP1 (phospho Y564) antibody (ab79294) at 1/1000 dilution
Lane 1 : extracts from COS7 cells, treated with EGF (200 ng/ml, 30 mins)
Lane 2 : extracts from COS7 cells, treated with EGF (200 ng/ml, 30 mins) with immunising peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 68 kDa