Antibodies, Proteins, Kits and Reagents for Life Science

291 484 ₸ Product size

100 µl 291 484 ₸

Order now and get it on Tuesday March 09, 2021

Anti-VILIP1 antibody [EPR9832] (ab180141)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR9832] to VILIP1
Suitable for: IHC-P, WB, Flow Cyt, IP
Reacts with: Mouse, Rat, Human

Product name

Anti-VILIP1 antibody [EPR9832]
See all VILIP1 primary antibodies
Description

Rabbit monoclonal [EPR9832] to VILIP1
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
IHC-P	Human
IP	Human
WB	Mouse Human

See all applications and species data

Immunogen

Synthetic peptide within Human VILIP1 aa 1-100. The exact sequence is proprietary.
Database link: P62760
Positive control
- Human cerebellum, Mouse brain and rat brain lysate, Human brain tissue, SH-SY5Y cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine)
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR9832
Isotype

IgG
Research areas

Western blot - Anti-VILIP1 antibody [EPR9832] (ab180141)

All lanes : Anti-VILIP1 antibody [EPR9832] (ab180141) at 1/1000 dilution

Lane 1 : Mouse brain lysate
Lane 2 : Rat brain lysate

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 22 kDa
Observed band size: 18 kDa
why is the actual band size different from the predicted?

Blocking buffer and concentration: 5% NFDM/TBST
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VILIP1 antibody [EPR9832] (ab180141)

Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling VILIP1 with ab180141 at 1/500 dilution. Secondary antibody Goat anti rabbit IgG(HRP) prediluted. Counter stain Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
Flow Cytometry - Anti-VILIP1 antibody [EPR9832] (ab180141)

Flow cytometric analysis of 2% paraformaldehyde fixed SH-SY5Y cells labeling VILIP1 with ab180141 at 1/50. Secondary antibody Goat anti rabbit IgG (FITC) at 1/75. Isotype control Rabbit IgG.
Immunoprecipitation - Anti-VILIP1 antibody [EPR9832] (ab180141)

Immunoprecipitation analysis of Human cerebellum lysate labeling VILIP1 using ab180141 at 1/60 dilution. A Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 was used as secondary antibody.
Western blot - Anti-VILIP1 antibody [EPR9832] (ab180141)

Anti-VILIP1 antibody [EPR9832] (ab180141) at 1/1000 dilution + Human cerebellum at 10 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab136636) at 1/500 dilution (Goat Anti-Rabbit IgG HRP)

Predicted band size: 22 kDa
Observed band size: 18 kDa why is the actual band size different from the predicted?

Blocking buffer and concentration: 5% NFDM/TBST
Anti-VILIP1 antibody [EPR9832] (ab180141)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com