Anti-liver Arginase antibody (ab91279)
Key features and details
- Rabbit polyclonal to liver Arginase
- Suitable for: ICC/IF, WB, IP
- Reacts with: Mouse, Rat
- Isotype: IgG
Overview
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Product name
Anti-liver Arginase antibody
See all Liver Arginase primary antibodies -
Description
Rabbit polyclonal to liver Arginase -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WB, IPmore details -
Species reactivity
Reacts with: Mouse, Rat
Predicted to work with: Pig
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Immunogen
Synthetic peptide corresponding to Mouse Liver Arginase aa 300 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available asab100829) -
Positive control
- WB: Rat & mouse liver tissue lysate. IP: Mouse liver tissue. ICC/IF: PC-12 cells.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab91279 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes ICC/IF Use a concentration of 5 µg/ml. WB Use a concentration of 1 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 35 kDa). IP Use a concentration of 5 µg/ml. Target
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Pathway
Nitrogen metabolism; urea cycle; L-ornithine and urea from L-arginine: step 1/1. -
Involvement in disease
Defects in ARG1 are the cause of argininemia (ARGIN) [MIM:207800]; also known as hyperargininemia. Argininemia is a rare autosomal recessive disorder of the urea cycle. Arginine is elevated in the blood and cerebrospinal fluid, and periodic hyperammonemia occurs. Clinical manifestations include developmental delay, seizures, mental retardation, hypotonia, ataxia, progressive spastic quadriplegia. -
Sequence similarities
Belongs to the arginase family. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 11846 Mouse
- Entrez Gene: 29221 Rat
- SwissProt: Q61176 Mouse
- SwissProt: P07824 Rat
- Unigene: 154144 Mouse
- Unigene: 9857 Rat
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Alternative names
- A I antibody
- Al antibody
- ARG 1 antibody
see all
Images
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Western blot - Anti-liver Arginase antibody (ab91279)All lanes : Anti-liver Arginase antibody (ab91279) at 1 µg/ml
Lane 1 : Liver (Mouse) Tissue Lysate
Lane 2 : Liver (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 35 kDa
Observed band size: 37,40 kDa why is the actual band size different from the predicted?
Exposure time: 30 seconds -
Immunoprecipitation - Anti-liver Arginase antibody (ab91279)Liver Arginase was immunoprecipitated using 0.5mg mouse liver tissue lysate, 5µg of Rabbit polyclonal to Liver Arginase and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, mouse liver tissue lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab91279.
Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
Band: 37, 40kDa: liver Arginase -
Immunocytochemistry/ Immunofluorescence - Anti-liver Arginase antibody (ab91279)ab91279 staining liver Arginase in PC-12 (rat adrenal gland pheochromocytoma cell line) cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with the antibody ab91279 at 5µg/ml and ab7291 (Mouse monoclonal to alpha Tubulin - Loading Control) used at a 1/1000 dilution overnight at +4°C. The secondary antibodies were ab150081, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed, (pseudo-colored green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594) preadsorbed, (colored red), both used at a 1/1000 dilution for 1 hour at room temperature. DAPI was used to stain the cell nuclei (colored blue) at a concentration of 1.43 µM for 1hour at room temperature.
Protocols
References (31)
ab91279 has been referenced in 31 publications.
- Bloomer SA et al. Aging results in accumulation of M1 and M2 hepatic macrophages and a differential response to gadolinium chloride. Histochem Cell Biol 153:37-48 (2020). IHC-P ; Rat . PubMed: 31691025
- Li R et al. Bone marrow mesenchymal stem cell-derived exosomal microRNA-124-3p attenuates neurological damage in spinal cord ischemia-reperfusion injury by downregulating Ern1 and promoting M2 macrophage polarization. Arthritis Res Ther 22:75 (2020). PubMed: 32272965
- McKelvey KJ et al. Temporal and spatial modulation of the tumor and systemic immune response in the murine Gl261 glioma model. PLoS One 15:e0226444 (2020). PubMed: 32240177
- Gaba RC et al. Development and comprehensive characterization of porcine hepatocellular carcinoma for translational liver cancer investigation. Oncotarget 11:2686-2701 (2020). PubMed: 32733642
- Feng Y et al. Tanshinone IIA attenuates demyelination and promotes remyelination in A. cantonensis-infected BALB/c mice. Int J Biol Sci 15:2211-2223 (2019). PubMed: 31592236
Images
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Western blot - Anti-liver Arginase antibody (ab91279)All lanes : Anti-liver Arginase antibody (ab91279) at 1 µg/ml
Lane 1 : Liver (Mouse) Tissue Lysate
Lane 2 : Liver (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 35 kDa
Observed band size: 37,40 kDa why is the actual band size different from the predicted?
Exposure time: 30 seconds
-
Immunoprecipitation - Anti-liver Arginase antibody (ab91279)
Liver Arginase was immunoprecipitated using 0.5mg mouse liver tissue lysate, 5µg of Rabbit polyclonal to Liver Arginase and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, mouse liver tissue lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab91279.
Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
Band: 37, 40kDa: liver Arginase -
Immunocytochemistry/ Immunofluorescence - Anti-liver Arginase antibody (ab91279)
ab91279 staining liver Arginase in PC-12 (rat adrenal gland pheochromocytoma cell line) cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with the antibody ab91279 at 5µg/ml and ab7291 (Mouse monoclonal to alpha Tubulin - Loading Control) used at a 1/1000 dilution overnight at +4°C. The secondary antibodies were ab150081, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed, (pseudo-colored green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594) preadsorbed, (colored red), both used at a 1/1000 dilution for 1 hour at room temperature. DAPI was used to stain the cell nuclei (colored blue) at a concentration of 1.43 µM for 1hour at room temperature.
