Antibodies, Proteins, Kits and Reagents for Life Science

459 004 ₸ Product size

1 ml 459 004 ₸ 500 µl 381 945 ₸

Order now and get it on Tuesday March 02, 2021

Anti-Liver Arginase antibody [SP156] (ab183333)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [SP156] to Liver Arginase
Suitable for: WB, IHC-P
Reacts with: Mouse, Human

Product name

Anti-Liver Arginase antibody [SP156]
See all Liver Arginase primary antibodies
Description

Rabbit monoclonal [SP156] to Liver Arginase
Host species

Rabbit
Tested Applications & Species

Application Species
IHC-P
Human

WB
Mouse

Human

See all applications and species data
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- IHC-P: Human liver, hepatocellular carcinoma, and liver cirrhosis tissue; WB: Mouse liver and human liver.
General notes
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.

Application	Species
IHC-P	Human
WB	Mouse Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA
Concentration information loading...
Purity

Protein A/G purified
Purification notes

Purified from TCS by protein A/G.
Clonality

Monoclonal
Clone number

SP156
Isotype

IgG
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Liver Arginase antibody [SP156] (ab183333)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human hepatocyte cancer tissue sections labeling Liver Arginase with Purified ab183333 at 1/100 dilution (0.54 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
Western blot - Anti-Liver Arginase antibody [SP156] (ab183333)

All lanes : Anti-Liver Arginase antibody [SP156] (ab183333) at 1.866 µg/ml

Lane 1 : Mouse liver tissue
Lane 2 : Mouse spleen tissue
Lane 3 : Human liver tissue
Lane 4 : Human spleen tissue

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 0.05 µg/ml

Predicted band size: 35 kDa
Observed band size: 37 kDa
why is the actual band size different from the predicted?

Exposure time:
Lane1 & 2 is 3min
Lane 3 & 4 is 4 s
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Liver Arginase antibody [SP156] (ab183333)

Immunohistochemical analysis of paraffin-embedded Human hepatocellular carcinoma tissue labeling Liver Arginase with ab183333 at 1/100 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Liver Arginase antibody [SP156] (ab183333)

Immunohistochemical analysis of paraffin-embedded Human hepatocellular carcinoma tissue labeling Liver Arginase with ab183333 at 1/100 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Liver Arginase antibody [SP156] (ab183333)

Immunohistochemical analysis of paraffin-embedded Human hepatocellular carcinoma tissue labeling Liver Arginase with ab183333 at 1/100 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Liver Arginase antibody [SP156] (ab183333)

Immunohistochemical analysis of paraffin-embedded Human hepatocellular carcinoma tissue labeling Liver Arginase with ab183333 at 1/100 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Liver Arginase antibody [SP156] (ab183333)

Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling Liver Arginase with ab183333 at 1/100 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Liver Arginase antibody [SP156] (ab183333)

Immunohistochemical analysis of paraffin-embedded Human liver cirrhosis tissue labeling Liver Arginase with ab183333 at 1/100 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Liver Arginase antibody [SP156] (ab183333)

Immunohistochemical analysis of paraffin-embedded Human hepatocellular carcinoma tissue labeling Liver Arginase with ab183333 at 1/100 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Liver Arginase antibody [SP156] (ab183333)

Immunohistochemical analysis of paraffin-embedded Human liver cirrhosis tissue labeling Liver Arginase with ab183333 at 1/100 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Liver Arginase antibody [SP156] (ab183333)

Immunohistochemical analysis of paraffin-embedded Human hepatocellular carcinoma tissue labeling Liver Arginase with ab183333 at 1/100 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Liver Arginase antibody [SP156] (ab183333)

Immunohistochemical analysis of paraffin-embedded Human hepatocellular carcinoma tissue labeling Liver Arginase with ab183333 at 1/100 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Liver Arginase antibody [SP156] (ab183333)

Immunohistochemical analysis of paraffin-embedded Human hepatocellular carcinoma tissue labeling Liver Arginase with ab183333 at 1/100 dilution.
Anti-Liver Arginase antibody [SP156] (ab183333)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com