Anti-Hydroxyproline antibody (ab37067)
Key features and details
- Rabbit polyclonal to Hydroxyproline
- Suitable for: IHC-Fr, WB
- Reacts with: Species independent
- Isotype: IgG
Overview
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Product name
Anti-Hydroxyproline antibody -
Description
Rabbit polyclonal to Hydroxyproline -
Host species
Rabbit -
Specificity
This antibody targets conjugated Trans-Hydroxyproline, it does not recognize free Trans-Hydroxyproline. -
Tested applications
Suitable for: IHC-Fr, WBmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Synthetic peptide corresponding to Hydroxyproline conjugated to bovine serum albumin.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
Constituents: 50% Glycerol, ddH20 -
Concentration information loading... -
Purity
Preadsorbed antiserum -
Purification notes
Antibody is purified via a salt cut, and then BSA antibodies are pre-adsorbed away. Antisera antibody was lyophilized and then reconstituted in buffer. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Hydroxyproline antibody (ab37067) Image courtesy of an anonymous Abreview.Anti-Hydroxyproline antibody (ab37067) at 1/500 dilution + lysate prepared from HEK293T cells at 1 µg
Secondary
HRP-conjugated goat anti-rabbit polyclonal at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Observed band size: 90 kDa why is the actual band size different from the predicted?
Exposure time: 2 minutes
WT flag tagged HIF1a was transfected into HEK293T cells. After 48 hours cells were treated with MG132 or DMSO for 6 hours. Cell lysates were subjected to Immunoprecipitation. Samples were separated in SDS-PAGE for Western blotting.Negative control: Flag tagged HIF1a mutant (Proline mutants).
