Anti-PNUTS antibody (ab70248)
Key features and details
- Rabbit polyclonal to PNUTS
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PNUTS antibody
See all PNUTS primary antibodies -
Description
Rabbit polyclonal to PNUTS -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Other Immunogen Type corresponding to PNUTS (C terminal). A region between residue 900 and the C-terminus (residue 940) of human PNUTS.
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Positive control
- Nuclear extract of Hela cells
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General notes
This product was previously labelled as PPP1R10
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Antibody was affinity purified using an epitope specific to PNUTS immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-PNUTS antibody (ab70248)Lanes 1-2 : Anti-PNUTS antibody (ab70248) at 1 µg/ml
Lanes 3-4 : Anti-PNUTS antibody (ab70248) at 0.2 µg/ml
Lanes 1 & 3 : Nuclear extracts from Hela cells at 15 µg
Lanes 2 & 4 : Nuclear extracts from Hela cells at 45 µg
Predicted band size: 99 kDa
Observed band size: 115 kDa why is the actual band size different from the predicted?
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Immunoprecipitation - Anti-PNUTS antibody (ab70248)Immunoprecipitation/ Western Blot of PNUTS Lane 1: Another antibody which recognizes a different epitope of PNUTS. Lane 2: Another antibody which recognizes a different epitope of PNUTS. Lane 3: Another antibody which recognizes a different epitope of PNUTS. Lane 4: ab70248 at 1µg/100µg nuclear extract. Detection: Chemiluminescence with exposure times of 3 seconds.
