Anti-PNUTS antibody (ab74589)
Key features and details
- Rabbit polyclonal to PNUTS
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PNUTS antibody
See all PNUTS primary antibodies -
Description
Rabbit polyclonal to PNUTS -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide corresponding to Human PNUTS aa 450-550.
(Peptide available asab74588) -
Positive control
- This antibody gave a positive signal in the following Nuclear Lysates: HeLa, HepG2, Jurkat.
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General notes
This product was previously labelled as PPP1R10
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab74589 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB HumanAll applications MouseRatApplication Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 110 kDa (predicted molecular weight: 99 kDa).Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 110 kDa (predicted molecular weight: 99 kDa).Target
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Function
Scaffold protein which mediates the formation of the PTW/PP1 phosphatase complex by providing a binding platform to each component of the complex. The PTW/PP1 phosphatase complex plays a role in the control of chromatin structure and cell cycle progression during the transition from mitosis into interphase. Mediates interaction of WDR82 and PPP1CA. Inhibitor of PPP1CA and PPP1CC phosphatase activities. Has inhibitory activity on PPP1CA only when phosphorylated. Binds to mRNA, single-stranded DNA (ssDNA), poly(A) and poly(G) homopolymers. -
Tissue specificity
Expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. -
Sequence similarities
Contains 1 C3H1-type zinc finger.
Contains 1 TFIIS N-terminal domain. -
Post-translational
modificationsPhosphorylated on Ser-398 by PKA within the region necessary for interaction with PPP1CA. -
Cellular localization
Nucleus. Found in discrete nucleoplasmic bodies and within nucleoli. Associates with chromatin during interphase, excluded from condensed chromosomes during early mitosis and is reloaded onto chromosomes at the late telophase (By similarity). - Information by UniProt
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Database links
- Entrez Gene: 5514 Human
- Entrez Gene: 52040 Mouse
- Entrez Gene: 65045 Rat
- Omim: 603771 Human
- SwissProt: Q96QC0 Human
- SwissProt: Q80W00 Mouse
- SwissProt: O55000 Rat
- Unigene: 106019 Human
see all -
Alternative names
- CAT 53 antibody
- CAT53 antibody
- FB 19 antibody
see all
Images
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Western blot - Anti-PNUTS antibody (ab74589)All lanes : Anti-PNUTS antibody (ab74589) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
Lane 2 : HepG2 (Human epithelial carcinoma cell line) Nuclear Lysate
Lane 3 : Jurkat nuclear extract lysate (ab14844)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 99 kDa
Observed band size: 110 kDa why is the actual band size different from the predicted?
Exposure time: 4 minutesPNUTS contains a number of potential phosphorylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted. The 110 kDa band observed is comparable to the molecular weight seen with other commercially available antibodies to this target.
Datasheets and documents
References (0)
ab74589 has not yet been referenced specifically in any publications.
Images
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Western blot - Anti-PNUTS antibody (ab74589)All lanes : Anti-PNUTS antibody (ab74589) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
Lane 2 : HepG2 (Human epithelial carcinoma cell line) Nuclear Lysate
Lane 3 : Jurkat nuclear extract lysate (ab14844)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 99 kDa
Observed band size: 110 kDa why is the actual band size different from the predicted?
Exposure time: 4 minutesPNUTS contains a number of potential phosphorylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted. The 110 kDa band observed is comparable to the molecular weight seen with other commercially available antibodies to this target.
