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Cancer Tumor biomarkers Enzymes Phosphatases

Anti-PPP2R2D antibody [EPR13624] (ab181071)

Price and availability

291 484 ₸

Availability

Order now and get it on Thursday March 04, 2021

Anti-PPP2R2D antibody [EPR13624] (ab181071)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13624] to PPP2R2D
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human

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Overview

  • Product name

    Anti-PPP2R2D antibody [EPR13624]
    See all PPP2R2D primary antibodies
  • Description

    Rabbit monoclonal [EPR13624] to PPP2R2D
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    WB
    Mouse
    Rat
    Human
    See all applications and species data
  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • HeLa, SH-SY5Y and Human fetal brain lysates.
  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR13624
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Cell Cycle
    • Kinases/Phosphatases
    • Phosphatases
    • Signal Transduction
    • Protein Phosphorylation
    • Ser / Thr Phosphatases

Images

  • Western blot - Anti-PPP2R2D antibody [EPR13624] (ab181071)
    Western blot - Anti-PPP2R2D antibody [EPR13624] (ab181071)
    Anti-PPP2R2D antibody [EPR13624] (ab181071) at 1/5000 dilution (purified) + Rat brain tissue lysate at 20 µg

    Secondary
    HRP conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 52 kDa
    Observed band size: 52 kDa



    Blocking Buffer: 5% NFDM/TBST

    Dilution Buffer: 5% NFDM/TBST

  • Western blot - Anti-PPP2R2D antibody [EPR13624] (ab181071)
    Western blot - Anti-PPP2R2D antibody [EPR13624] (ab181071)
    Anti-PPP2R2D antibody [EPR13624] (ab181071) at 1/5000 dilution (purified) + Mouse brain tissue lysate

    Secondary
    HRP conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 52 kDa
    Observed band size: 52 kDa



    Blocking Buffer: 5% NFDM/TBST

    Dilution Buffer: 5% NFDM/TBST

  • Western blot - Anti-PPP2R2D antibody [EPR13624] (ab181071)
    Western blot - Anti-PPP2R2D antibody [EPR13624] (ab181071)
    All lanes : Anti-PPP2R2D antibody [EPR13624] (ab181071) at 1/1000 dilution (purified)

    Lane 1 : SH-SY5Y cell lysate
    Lane 2 : Human fetal brain tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : HRP conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 52 kDa
    Observed band size: 52 kDa



    Blocking Buffer: 5% NFDM/TBST

    Dilution Buffer: 5% NFDM/TBST

  • Western blot - Anti-PPP2R2D antibody [EPR13624] (ab181071)
    Western blot - Anti-PPP2R2D antibody [EPR13624] (ab181071)
    All lanes : Anti-PPP2R2D antibody [EPR13624] (ab181071) at 1/2000 dilution (unpurified)

    Lane 1 : HeLa cell lysate
    Lane 2 : SH-SY5Y cell lysate
    Lane 3 : Human fetal brain lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 52 kDa
    Observed band size: 52 kDa

  • Anti-PPP2R2D antibody [EPR13624] (ab181071)
    Anti-PPP2R2D antibody [EPR13624] (ab181071)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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