Anti-PPP2R2D antibody [EPR13624] (ab181071)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13624] to PPP2R2D
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Overview
-
Product name
Anti-PPP2R2D antibody [EPR13624]
See all PPP2R2D primary antibodies -
Description
Rabbit monoclonal [EPR13624] to PPP2R2D -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB MouseRatHuman -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- HeLa, SH-SY5Y and Human fetal brain lysates.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR13624 -
Isotype
IgG -
Research areas
Images
-
Anti-PPP2R2D antibody [EPR13624] (ab181071) at 1/5000 dilution (purified) + Rat brain tissue lysate at 20 µg
Secondary
HRP conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 52 kDa
Observed band size: 52 kDaBlocking Buffer: 5% NFDM/TBST
Dilution Buffer: 5% NFDM/TBST
-
Anti-PPP2R2D antibody [EPR13624] (ab181071) at 1/5000 dilution (purified) + Mouse brain tissue lysate
Secondary
HRP conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 52 kDa
Observed band size: 52 kDaBlocking Buffer: 5% NFDM/TBST
Dilution Buffer: 5% NFDM/TBST
-
All lanes : Anti-PPP2R2D antibody [EPR13624] (ab181071) at 1/1000 dilution (purified)
Lane 1 : SH-SY5Y cell lysate
Lane 2 : Human fetal brain tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : HRP conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 52 kDa
Observed band size: 52 kDaBlocking Buffer: 5% NFDM/TBST
Dilution Buffer: 5% NFDM/TBST
-
All lanes : Anti-PPP2R2D antibody [EPR13624] (ab181071) at 1/2000 dilution (unpurified)
Lane 1 : HeLa cell lysate
Lane 2 : SH-SY5Y cell lysate
Lane 3 : Human fetal brain lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 52 kDa
Observed band size: 52 kDa
-