Anti-PPP2R2D antibody (ab229863)
Key features and details
- Rabbit polyclonal to PPP2R2D
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PPP2R2D antibody
See all PPP2R2D primary antibodies -
Description
Rabbit polyclonal to PPP2R2D -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-P, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Chicken, Xenopus laevis, Zebrafish, Xenopus tropicalis
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Immunogen
Recombinant fragment corresponding to Human PPP2R2D aa 1-200.
Database link: Q66LE6 -
Positive control
- WB: SH-SY5Y, HeLa and HEK-293T whole cell lysates. IHC-P: Human prostate cancer and glioma cancer tissues. ICC/IF: HeLa cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300 -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purity >95%. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-PPP2R2D antibody (ab229863)All lanes : Anti-PPP2R2D antibody (ab229863) at 1/1000 dilution
Lane 1 : SH-SY5Y (human neuroblastoma cell line from bone marrow) whole cell lysate
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 3 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Secondary
All lanes : Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 52 kDa
Observed band size: 52,40 kDa why is the actual band size different from the predicted?
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Immunocytochemistry/ Immunofluorescence - Anti-PPP2R2D antibody (ab229863)HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for PPP2RD (green) using ab229863 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L) secondary antibody.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPP2R2D antibody (ab229863)Paraffin-embedded human glioma tissue stained for PPP2RD using ab229863 at 1/100 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPP2R2D antibody (ab229863)Paraffin-embedded human prostate cancer tissue stained for PP2R2D using ab229863 at 1/100 dilution in immunohistochemical analysis.
