Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-SA2 antibody [EPR17865] - C-terminal (ab201451)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR17865] to SA2 - C-terminal
  • Suitable for: Flow Cyt, WB, IHC-P, ICC/IF
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-SA2 antibody [EPR17865] - C-terminal
    See all SA2 primary antibodies

  • Description

    Rabbit monoclonal [EPR17865] to SA2 - C-terminal

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Mouse
    Rat
    Human
    WB
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa, HCT116, MCF-7, K562, C6, Raw264.7 and NIH3T3 cell lysates; Human fetal brain and Mouse spleen lysates; IHC-P: Human breast carcinoma, human tonsil, mouse and rat spleen tissue; IF: MCF-7 and K562 cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-SA2 antibody [EPR17865] - C-terminal (ab201451)
    Western blot - Anti-SA2 antibody [EPR17865] - C-terminal (ab201451)
    All lanes : Anti-SA2 antibody [EPR17865] - C-terminal (ab201451) at 1/1000 dilution

    Lane 1 : Wild-type HeLa cell lysate
    Lane 2 : STAG2 knockout HeLa cell lysate
    Lane 3 : HCT116 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

    Predicted band size: 141 kDa
    Observed band size: 141 kDa



    Lanes 1-3: Merged signal (red and green). Green - ab201451 observed at 141 kDa. Red - loading control ab8245 observed at 36 kDa.

    ab201451 Anti-SA2 antibody [EPR17865] - C-terminal was shown to specifically react with SA2 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265461 (knockout cell lysate ab257707) was used. Wild-type and SA2 knockout samples were subjected to SDS-PAGE. ab201451 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

     

  • Flow Cytometry - Anti-SA2 antibody [EPR17865] - C-terminal (ab201451)
    Flow Cytometry - Anti-SA2 antibody [EPR17865] - C-terminal (ab201451)

    Flow Cytometry analysis of K562(human chronic myelogenous leukemia) labelling SA2 with purified ab201451 at 1/25000 (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Alexa Fluor® 488 goat anti-rabbit IgG (1/2000) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.

     

  • Western blot - Anti-SA2 antibody [EPR17865] - C-terminal (ab201451)
    Western blot - Anti-SA2 antibody [EPR17865] - C-terminal (ab201451)
    All lanes : Anti-SA2 antibody [EPR17865] - C-terminal (ab201451) at 1/10000 dilution

    Lane 1 : MCF-7 (Human breast adenocarcinoma cell line) cell lysate
    Lane 2 : K562 (Human chronic myelogenous leukemia cells from bone marrow) cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 141 kDa
    Observed band size: 141 kDa


    Exposure time: 3 minutes


    5% NFDM/TBST: Blocking and diluting buffer.

  • Western blot - Anti-SA2 antibody [EPR17865] - C-terminal (ab201451)
    Western blot - Anti-SA2 antibody [EPR17865] - C-terminal (ab201451)
    Anti-SA2 antibody [EPR17865] - C-terminal (ab201451) at 1/1000 dilution + Human fetal brain lysate at 10 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 141 kDa
    Observed band size: 141 kDa


    Exposure time: 1 minute


    5% NFDM/TBST: Blocking and diluting buffer.

  • Western blot - Anti-SA2 antibody [EPR17865] - C-terminal (ab201451)
    Western blot - Anti-SA2 antibody [EPR17865] - C-terminal (ab201451)
    All lanes : Anti-SA2 antibody [EPR17865] - C-terminal (ab201451) at 1/10000 dilution

    Lane 1 : C6 (Rat glial tumor cells) cell lysate
    Lane 2 : Raw264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) cell lysate
    Lane 3 : NIH 3T3 (Mouse embyro fibroblast cells) cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 141 kDa
    Observed band size: 141 kDa


    Exposure time: 3 minutes


    5% NFDM/TBST: Blocking and diluting buffer.

  • Western blot - Anti-SA2 antibody [EPR17865] - C-terminal (ab201451)
    Western blot - Anti-SA2 antibody [EPR17865] - C-terminal (ab201451)
    Anti-SA2 antibody [EPR17865] - C-terminal (ab201451) at 1/1000 dilution + mouse spleen lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 141 kDa
    Observed band size: 141 kDa


    Exposure time: 1 minute


    5% NFDM/TBST: Blocking and diluting buffer.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SA2 antibody [EPR17865] - C-terminal (ab201451)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SA2 antibody [EPR17865] - C-terminal (ab201451)

    Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling SA2 using ab201451 at 1/2000 dilution. A Goat Anti-Rabbit IgG H&L (HRP) (ab97051) was used as secondary at 1/500 dilution. Counterstain: Hematoxylin.
    Inset image: negative control obtained using PBS instead of ab201451, and secondary antibody only.
    Note: Nuclear staining on Human breast carcinoma tissue was observed.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SA2 antibody [EPR17865] - C-terminal (ab201451)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SA2 antibody [EPR17865] - C-terminal (ab201451)

    Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling SA2 using ab201451 at 1/2000 dilution. A Goat Anti-Rabbit IgG H&L (HRP) (ab97051) was used as secondary at 1/500 dilution. Counterstain: Hematoxylin.
    Inset image: negative control obtained using PBS instead of ab201451, and secondary antibody.
    Note: Nuclear staining on Human tonsil tissue was observed.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SA2 antibody [EPR17865] - C-terminal (ab201451)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SA2 antibody [EPR17865] - C-terminal (ab201451)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling SA2 using ab201451 at 1/2000 dilution. A Goat Anti-Rabbit IgG H&L (HRP) (ab97051) was used as secondary at 1/500 dilution. Counterstain: Hematoxylin.
    Inset image: negative control obtained using PBS instead of ab201451, and secondary antibody.
    Note: Nuclear staining on mouse spleen tissue was observed.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SA2 antibody [EPR17865] - C-terminal (ab201451)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SA2 antibody [EPR17865] - C-terminal (ab201451)

    Immunohistochemical analysis of paraffin-embedded rat spleen tissue labeling SA2 using ab201451 at 1/2000 dilution. A Goat Anti-Rabbit IgG H&L (HRP) (ab97051) was used as secondary at 1/500 dilution. Counterstain: Hematoxylin.
    Inset image: negative control obtained using PBS instead of ab201451, and secondary antibody.
    Note: Nuclear staining on rat spleen tissue was observed.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-SA2 antibody [EPR17865] - C-terminal (ab201451)
    Immunocytochemistry/ Immunofluorescence - Anti-SA2 antibody [EPR17865] - C-terminal (ab201451)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MCF7 (Human breast adenocarcinoma cell line) cells labeling SA2 with ab201451 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green).

    Confocal image showing nuclear staining on MCF7 cell line.

    The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
    The negative controls are as follows:
    1. ab201451 at 1/500 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
    2. ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-SA2 antibody [EPR17865] - C-terminal (ab201451)
    Immunocytochemistry/ Immunofluorescence - Anti-SA2 antibody [EPR17865] - C-terminal (ab201451)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized K562 (Human chronic myelogenous leukemia cells from bone marrow) cells labeling SA2 with ab201451 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green).

    Confocal image showing nuclear staining on K562 cell line.

    The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
    The negative controls are as follows:
    1. ab201451 at 1/500 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
    2. ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.

  • Anti-SA2 antibody [EPR17865] - C-terminal (ab201451)
    Anti-SA2 antibody [EPR17865] - C-terminal (ab201451)

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