Anti-SA2 antibody - C-terminal (ab229609)
Key features and details
- Rabbit polyclonal to SA2 - C-terminal
- Suitable for: WB, IP, ICC/IF
- Reacts with: Human, Recombinant fragment
- Isotype: IgG
Overview
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Product name
Anti-SA2 antibody - C-terminal
See all SA2 primary antibodies -
Description
Rabbit polyclonal to SA2 - C-terminal -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIP HumanWB HumanRecombinant fragment
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Immunogen
Synthetic peptide within Human SA2 (C terminal). The exact sequence is proprietary. Carrier-protein conjugated
Database link: Q8N3U4 -
Positive control
- WB: HEK-293T, A431, HeLa and HepG2 whole cell extracts. IP: HeLa whole cell extract. ICC/IF: HeLa cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-SA2 antibody - C-terminal (ab229609)All lanes : Anti-SA2 antibody - C-terminal (ab229609) at 1/6000 dilution
Lane 1 : Non-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
Lane 2 : SA2 shRNA-transfected HEK-293T whole cell extract
Lysates/proteins at 30 µg per lane.
Developed using the ECL technique.
Predicted band size: 141 kDa5% SDS-PAGE gel.
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Immunocytochemistry/ Immunofluorescence - Anti-SA2 antibody - C-terminal (ab229609)4% paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for SA2 (green) using ab229609 1/500 dilution in ICC/IF.
Nuclear counterstain: Hoechst 33342 (blue). F-actin stained with Phalloidin (red).
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Western blot - Anti-SA2 antibody - C-terminal (ab229609)All lanes : Anti-SA2 antibody - C-terminal (ab229609) at 1/1000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell extract
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract
Lysates/proteins at 30 µg per lane.
Developed using the ECL technique.
Predicted band size: 141 kDa5% SDS-PAGE gel.
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Immunoprecipitation - Anti-SA2 antibody - C-terminal (ab229609)SA2 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract with 5μg ab229609. Western blot was performed from the immunoprecipitate using ab229609.
Lane 1: Control IgG instead of ab229609 in HeLa whole cell extract.
Lane 2: ab229609 IP in HeLa whole cell extract.
