Antibodies, Proteins, Kits and Reagents for Life Science

298 185 ₸ Product size

100 µl 298 185 ₸

Order now and get it on Tuesday March 02, 2021

Anti-SA2 antibody [EPR10994(B)] (ab155081)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR10994(B)] to SA2
Suitable for: WB, IHC-P
Knockout validated
Reacts with: Human

Product name

Anti-SA2 antibody [EPR10994(B)]
See all SA2 primary antibodies
Description

Rabbit monoclonal [EPR10994(B)] to SA2
Host species

Rabbit
Tested Applications & Species

Application Species
IHC-P
Human

WB
Human

See all applications and species data
Immunogen

Synthetic peptide within Human SA2 aa 1200 to the C-terminus. The exact sequence is proprietary.
Database link: Q8N3U4
Positive control
- WB: HCT116, HeLa, K562, MCF7 and Jurkat whole cell lysate (ab7899). IHC-P: Human colon tissue and gastric adenocarcinoma tissue.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
IHC-P	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20ºC.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR10994(B)
Isotype

IgG
Research areas

Western blot - Anti-SA2 antibody [EPR10994(B)] (ab155081)

All lanes : Anti-SA2 antibody [EPR10994(B)] (ab155081) at 1/500 dilution

Lane 1 : Wild-type HeLa cell lysate
Lane 2 : STAG2 knockout HeLa cell lysate
Lane 3 : HCT116 cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

Predicted band size: 141 kDa
Observed band size: 141 kDa

Lanes 1-3: Merged signal (red and green). Green - ab155081 observed at 141 kDa. Red - loading control ab8245 observed at 36 kDa.

ab155081 Anti-SA2 antibody [EPR10994(B)] was shown to specifically react with SA2 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265461 (knockout cell lysate ab257707) was used. Wild-type and SA2 knockout samples were subjected to SDS-PAGE. ab155081 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SA2 antibody [EPR10994(B)] (ab155081)

Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling SA2 with ab155081 at 1/50 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Western blot - Anti-SA2 antibody [EPR10994(B)] (ab155081)

All lanes : Anti-SA2 antibody [EPR10994(B)] (ab155081) at 1/10000 dilution

Lane 1 : HeLa cell lysate
Lane 2 : K562 cell lysate
Lane 3 : MCF7 cell lysate
Lane 4 : Jurkat cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 141 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SA2 antibody [EPR10994(B)] (ab155081)

Immunohistochemical analysis of paraffin-embedded Human gastric adenocarcinoma tissue labeling SA2 with ab155081 at 1/50 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SA2 antibody [EPR10994(B)] (ab155081)

Immunohistochemical analysis of paraffin embedded Human normal spleen tissue using ab155081 showing +ve staining.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SA2 antibody [EPR10994(B)] (ab155081)

Immunohistochemical analysis of paraffin embedded Human ovarian carcinoma tissue using ab155081 showing +ve staining.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SA2 antibody [EPR10994(B)] (ab155081)

Immunohistochemical analysis of paraffin embedded Human melanoma tissue using ab155081 showing +ve staining.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SA2 antibody [EPR10994(B)] (ab155081)

Immunohistochemical analysis of paraffin embedded Human normal breast tissue using ab155081 showing +ve staining.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Anti-SA2 antibody [EPR10994(B)] (ab155081)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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