Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Galectin 1 antibody [EPR3205] (ab108389)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3205] to Galectin 1
  • Suitable for: WB, ICC/IF
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Galectin 1 antibody [EPR3205]
    See all Galectin 1 primary antibodies

  • Description

    Rabbit monoclonal [EPR3205] to Galectin 1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Mouse
    Human
    WB
    Mouse
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HL60, K562, and NIH/3T3 cell lysates. Mouse heart, kidney, and spleen tissue lysates. Rat heart and kidney tissue lysates; ICC/IF: NIH/3T3 and K562 cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-Galectin 1 antibody [EPR3205] (ab108389)
    Western blot - Anti-Galectin 1 antibody [EPR3205] (ab108389)
    All lanes : Anti-Galectin 1 antibody [EPR3205] (ab108389) at 1/1000 dilution

    Lane 1 : HL60 cell lysate
    Lane 2 : K562 cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 15 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-Galectin 1 antibody [EPR3205] (ab108389)
    Immunocytochemistry/ Immunofluorescence - Anti-Galectin 1 antibody [EPR3205] (ab108389)

    Immunocytochemistry/immunofluorescence analysis of K562 (human chronic myelogenous leukemia cell line from bone marrow) cells labelling Galectin 1 with purified ab108389 at 1/100. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 was used as the secondary antibody (green). Cells were counterstained with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) at 1/200 dilution (red). Nuclear DNA was labelled with DAPI (blue).

  • Immunocytochemistry/ Immunofluorescence - Anti-Galectin 1 antibody [EPR3205] (ab108389)
    Immunocytochemistry/ Immunofluorescence - Anti-Galectin 1 antibody [EPR3205] (ab108389)

    Immunocytochemistry/immunofluorescence analysis of NIH/3T3 (mouse embryo fibroblast cell line) cells labelling Galectin 1 with purified ab108389 at 1/100. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 was used as the secondary antibody (green). Cells were counterstained with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) at 1/200 dilution (red). Nuclear DNA was labelled with DAPI (blue).

  • Western blot - Anti-Galectin 1 antibody [EPR3205] (ab108389)
    Western blot - Anti-Galectin 1 antibody [EPR3205] (ab108389)
    All lanes : Anti-Galectin 1 antibody [EPR3205] (ab108389) at 1/1000 dilution

    Lane 1 : mouse heart tissue lysate
    Lane 2 : mouse kidney tissue lysate
    Lane 3 : mouse spleen tissue lysate
    Lane 4 : rat heart tissue lysate
    Lane 5 : rat kidney tissue lysate
    Lane 6 : NIH 3T3 cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 15 kDa

  • Anti-Galectin 1 antibody [EPR3205] (ab108389)
    Anti-Galectin 1 antibody [EPR3205] (ab108389)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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