Rat IFN gamma ELISA Kit (ab239425)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 1.1 pg/ml
- Range: 7 pg/ml - 500 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Rat
Overview
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Product name
Rat IFN gamma ELISA Kit
See all Interferon gamma kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% splenocyte 8 3.6% Inter-assay Sample n Mean SD CV% splenocyte 3 5% -
Sample type
Cell culture supernatant, Serum, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
1.1 pg/ml -
Range
7 pg/ml - 500 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 100 93% - 106% Serum 97 93% - 104% Cell culture media 95 91% - 97% Hep Plasma 95 94% - 96% EDTA Plasma 122 120% - 127% Cit plasma 109 107% - 110% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Rat -
Product overview
Rat IFN gamma ELISA Kit (ab239425) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IFN gamma protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Rat IFN gamma with 1.1 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Rat IFN gamma Capture Antibody 1 x 600µl 10X Rat IFN gamma Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 4BR 1 x 6ml Plate Seals 1 unit Rat IFN gamma Lyophilized Recombinant Protein 2 vials Sample Diluent 75BP 1 x 20ml Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons. -
Tissue specificity
Released primarily from activated T lymphocytes. -
Involvement in disease
In Caucasians, genetic variation in IFNG is associated with the risk of aplastic anemia (AA) [MIM:609135]. AA is a rare disease in which the reduction of the circulating blood cells results from damage to the stem cell pool in bone marrow. In most patients, the stem cell lesion is caused by an autoimmune attack. T-lymphocytes, activated by an endogenous or exogenous, and most often unknown antigenic stimulus, secrete cytokines, including IFN-gamma, which would in turn be able to suppress hematopoiesis. -
Sequence similarities
Belongs to the type II (or gamma) interferon family. -
Post-translational
modificationsProteolytic processing produces C-terminal heterogeneity, with proteins ending alternatively at Gly-150, Met-157 or Gly-161. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- IF 1
- IFG
- IFI
see all -
Database links
- Entrez Gene: 25712 Rat
- SwissProt: P01581 Rat
- Unigene: 10795 Rat
Images
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Other - Rat IFN gamma ELISA Kit (ab239425)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Rat IFN gamma Standard Curve ComparisonStandard Curve comparison between Rat IFN gamma SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity. -
Example of rat IFN gamma standard curve in Sample Diluent NS.The IFN gamma standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed. -
Example of rat IFN gamma standard curve in Sample Diluent 75BP.The IFN gamma standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed. -
Interpolated concentrations of native IFN gamma in treated and untreated rat splenocyte cell culture supernatant samples.Rat splenocyte cells were cultured in the presence (treated) or absence (untreated) of 5 µg/mL of Concanavalin A for 48 hours. The concentrations of IFN gamma were measured in duplicates, interpolated from the IFN gamma standard curves and corrected for sample dilution. Undiluted samples are as follows: treated splenocyte supernatant 10% and untreated splenocyte supernatant 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IFN gamma concentration was determined to be 4074 pg/mL in neat treated splenocyte supernatant, and 17.27 pg/mL in neat untreated splenocyte supernatant.
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Sandwich ELISA - Rat IFN gamma ELISA Kit (ab239425)To learn more about the advantages of recombinant antibodies see here.
