Antibodies, Proteins, Kits and Reagents for Life Science

288 134 ₸ Product size

100 µl 288 134 ₸

Order now and get it on Tuesday March 09, 2021

Anti-DPP4 antibody [EPR18215] (ab187048)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR18215] to DPP4
Suitable for: WB, IHC-P, IHC-Fr
Reacts with: Mouse, Rat

Product name

Anti-DPP4 antibody [EPR18215]
See all DPP4 primary antibodies
Description

Rabbit monoclonal [EPR18215] to DPP4
Host species

Rabbit

Tested Applications & Species

Application	Species
IHC-Fr	Mouse Rat
IHC-P	Mouse Rat
WB	Mouse Rat

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Rat lung, thymus, liver and kidney tissue lysate; mouse lung and thymus tissue lysate. IHC-P: Mouse liver tissue; rat liver tissue. IHC-Fr: Mouse liver tissue; rat liver tissue.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), 59% PBS
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR18215
Isotype

IgG
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DPP4 antibody [EPR18215] (ab187048)

Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling CD26 with ab187048 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Membranous staining on bile canaliculi of mouse liver is observed (PMID: 23361237). Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Western blot - Anti-DPP4 antibody [EPR18215] (ab187048)

All lanes : Anti-DPP4 antibody [EPR18215] (ab187048) at 1/1000 dilution

Lane 1 : Mouse lung lysate
Lane 2 : Rat lung lysate
Lane 3 : Mouse thymus lysate
Lane 4 : Rat thymus lysate
Lane 5 : Rat liver lysate
Lane 6 : Rat kidney lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG H&L (HRP))

Predicted band size: 88 kDa
Observed band size: 110 kDa
why is the actual band size different from the predicted?

Blocking and dilution buffer: 5% NFDM/TBST

Exposure times.

Lanes 1, 3, 4: 30 seconds

Lane 2: 20 seconds

Lane 5: 10 seconds

Lane 6: 15 seconds

The molecular weight observed is consistent with what has been described in the literature (PMID: 25589660).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DPP4 antibody [EPR18215] (ab187048)

Immunohistochemical analysis of paraffin-embedded rat liver tissue labeling CD26 with ab187048 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Membranous staining on bile canaliculi of rat liver is observed (PMID: 23361237). Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Immunohistochemistry (Frozen sections) - Anti-DPP4 antibody [EPR18215] (ab187048)

Immunohistochemical analysis of 4% PFA-fixed, 0.2 % Triton X-100 permeabilized mouse liver tissue labeling CD26 with ab187048 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 dilution (green). Strong positive staining on the bile canaliculi and moderate positive staining in sinusoid endothelial cells is observed (PMID: 23361237). The nuclear counter stain is DAPI (blue)
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.

Antigen retrieval: Heated Tris-EDTA (pH9.0) (ab94681).
Immunohistochemistry (Frozen sections) - Anti-DPP4 antibody [EPR18215] (ab187048)

Immunohistochemical analysis of 4% PFA-fixed, 0.2 % Triton X-100 permeabilized rat liver tissue labeling CD26 with ab187048 at 1/200 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 dilution (green). Strong positive staining on the bile canaliculi and moderate positive staining in sinusoid endothelial cells is observed (PMID: 23361237). The nuclear counter stain is DAPI (blue)
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.

Antigen retrieval: Heated Tris-EDTA (pH9.0) (ab94681).
Anti-DPP4 antibody [EPR18215] (ab187048)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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