Anti-FIP200 antibody (ab227726)
Key features and details
- Rabbit polyclonal to FIP200
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-FIP200 antibody
See all FIP200 primary antibodies -
Description
Rabbit polyclonal to FIP200 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Human -
Immunogen
Recombinant fragment within Human FIP200 (N terminal). The exact sequence is proprietary.
Database link: Q8TDY2 -
Positive control
- WB: HEK-293T and A431 whole cell lysate; FIP200-transfected HEK-293T whole cell lysate. ICC/IF: HeLa cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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4% paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for FIP200 (green) using ab227726 at 1/200 dilution in ICC/IF.
Blue: Hoechst 33342 staining.
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All lanes : Anti-FIP200 antibody (ab227726) at 1/1000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 183 kDa5 % SDS-PAGE gel.
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All lanes : Anti-FIP200 antibody (ab227726) at 1/5000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : FIP200-transfected HEK-293T whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 183 kDa5 % SDS-PAGE gel.