Antibodies, Proteins, Kits and Reagents for Life Science

Key features and details

Rabbit polyclonal to FIP200
Suitable for: WB, ICC/IF
Reacts with: Human
Isotype: IgG

Product name

Anti-FIP200 antibody
See all FIP200 primary antibodies
Description

Rabbit polyclonal to FIP200
Host species

Rabbit
Tested Applications & Species

Application Species
ICC/IF
Human

WB
Human

See all applications and species data
Immunogen

Recombinant fragment within Human FIP200 (N terminal). The exact sequence is proprietary.
Database link: Q8TDY2
Positive control
- WB: HEK-293T and A431 whole cell lysate; FIP200-transfected HEK-293T whole cell lysate. ICC/IF: HeLa cells.

Application	Species
ICC/IF	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
Purity

Immunogen affinity purified
Clonality

Polyclonal
Isotype

IgG
Research areas

Immunocytochemistry/ Immunofluorescence - Anti-FIP200 antibody (ab227726)

4% paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for FIP200 (green) using ab227726 at 1/200 dilution in ICC/IF.

Blue: Hoechst 33342 staining.
Western blot - Anti-FIP200 antibody (ab227726)

All lanes : Anti-FIP200 antibody (ab227726) at 1/1000 dilution

Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell lysate

Lysates/proteins at 30 µg per lane.

Predicted band size: 183 kDa

5 % SDS-PAGE gel.
Western blot - Anti-FIP200 antibody (ab227726)

All lanes : Anti-FIP200 antibody (ab227726) at 1/5000 dilution

Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : FIP200-transfected HEK-293T whole cell lysate

Lysates/proteins at 30 µg per lane.

Predicted band size: 183 kDa

5 % SDS-PAGE gel.

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