Anti-FIP200 antibody (ab176816)
Key features and details
- Rabbit polyclonal to FIP200
- Suitable for: IP, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-FIP200 antibody
See all FIP200 primary antibodies -
Description
Rabbit polyclonal to FIP200 -
Host species
Rabbit -
Tested applications
Suitable for: IP, WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Common marmoset, Orangutan
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Immunogen
Synthetic peptide within Human FIP200 aa 1544-1594 (C terminal). The exact sequence is proprietary. NP_055596.3
Sequence:EGASGAS RRPWVLGKVM EKEYCQAKKA QNRFKVPLGT KFYRVKAVSW NKKV
Database link: Q8TDY2 -
Positive control
- HeLa, 293T and TCMK1 whole cell lysates.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 99% Tris buffered saline, 0.1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab176816 was affinity purified using an epitope specific to FIP200 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-FIP200 antibody (ab176816)All lanes : Anti-FIP200 antibody (ab176816) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysates at 50 µg
Lane 2 : HeLa whole cell lysates at 15 µg
Lane 3 : HeLa whole cell lysates at 5 µg
Lane 4 : 293T whole cell lysates at 50 µg
Developed using the ECL technique.
Predicted band size: 183 kDa
Exposure time: 30 seconds
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Western blot - Anti-FIP200 antibody (ab176816)All lanes : Anti-FIP200 antibody (ab176816) at 0.2 µg/ml
Lane 1 : TCMK1 whole cell lysates at 50 µg
Lane 2 : TCMK1 whole cell lysates at 15 µg
Developed using the ECL technique.
Predicted band size: 183 kDa
Exposure time: 3 minutes
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Immunoprecipitation - Anti-FIP200 antibody (ab176816)ab176816 at 0.1 µg/ml detecting FIP200 in HeLa whole cell lysate by WB following IP.
Lane 1: ab176816 at 3µg/mg of lysate
Lane 2: Control IgG.In each case, 1 mg of lysate was used for IP and 20% of the IP was loaded.
Detection: Chemiluminescence with an exposure time of 30 seconds.
