Antibodies, Proteins, Kits and Reagents for Life Science

304 886 ₸ Product size

100 µl 304 886 ₸

Order now and get it on Tuesday March 09, 2021

Anti-C4 antibody [EPR2990(2)] (ab173577)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR2990(2)] to C4
Suitable for: WB, ICC/IF, Flow Cyt
Reacts with: Human

Product name

Anti-C4 antibody [EPR2990(2)]
See all C4 primary antibodies
Description

Rabbit monoclonal [EPR2990(2)] to C4
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide within Human C4 aa 1200-1300 (Cysteine residue). The exact sequence is proprietary. Note: this sequence is identical in both C4 subunits A (P0C0L4) and B (P0C0L5).
Database link: P0C0L4
Positive control
- WB: HepG2 cell lysate. ICC/IF: HepG2 cells. Flow Cyt: HepG2 cells.
General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR2990(2)
Isotype

IgG
Research areas

Western blot - Anti-C4 antibody [EPR2990(2)] (ab173577)

Anti-C4 antibody [EPR2990(2)] (ab173577) at 1/1000 dilution + HepG2 cell lysate at 10 µg

Predicted band size: 193 kDa
Immunocytochemistry/ Immunofluorescence - Anti-C4 antibody [EPR2990(2)] (ab173577)

Immunofluorescent staining of HepG2 cells labeling C4 with ab173577 at 1/100
Flow Cytometry - Anti-C4 antibody [EPR2990(2)] (ab173577)

Flow cytometry analysis of HepG2 (human hepatocellular carcinoma) cells labeling C4 (red) with ab173577 at a 1/20 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluor^® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (ab172730). Blue (unlabeled control) - Cells without incubation with the primary and secondary antibodies.
Anti-C4 antibody [EPR2990(2)] (ab173577)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com