Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-C4 antibody [EPR2990(2)] (ab173577)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2990(2)] to C4
  • Suitable for: WB, ICC/IF, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-C4 antibody [EPR2990(2)]
    See all C4 primary antibodies

  • Description

    Rabbit monoclonal [EPR2990(2)] to C4

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human C4 aa 1200-1300 (Cysteine residue). The exact sequence is proprietary. Note: this sequence is identical in both C4 subunits A (P0C0L4) and B (P0C0L5).
    Database link: P0C0L4

  • Positive control

    • WB: HepG2 cell lysate. ICC/IF: HepG2 cells. Flow Cyt: HepG2 cells.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR2990(2)

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-C4 antibody [EPR2990(2)] (ab173577)
    Western blot - Anti-C4 antibody [EPR2990(2)] (ab173577)
    Anti-C4 antibody [EPR2990(2)] (ab173577) at 1/1000 dilution + HepG2 cell lysate at 10 µg

    Predicted band size: 193 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-C4 antibody [EPR2990(2)] (ab173577)
    Immunocytochemistry/ Immunofluorescence - Anti-C4 antibody [EPR2990(2)] (ab173577)

    Immunofluorescent staining of HepG2 cells labeling C4 with ab173577 at 1/100

  • Flow Cytometry - Anti-C4 antibody [EPR2990(2)] (ab173577)
    Flow Cytometry - Anti-C4 antibody [EPR2990(2)] (ab173577)

    Flow cytometry analysis of HepG2 (human hepatocellular carcinoma) cells labeling C4 (red) with ab173577 at a 1/20 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (ab172730). Blue (unlabeled control) - Cells without incubation with the primary and secondary antibodies.

  • Anti-C4 antibody [EPR2990(2)] (ab173577)
    Anti-C4 antibody [EPR2990(2)] (ab173577)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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