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Anti-C5b-9 + C5b-8 antibody [aE11] (ab66768)

Key features and details

  • Mouse monoclonal [aE11] to C5b-9 + C5b-8
  • Suitable for: IHC-Fr
  • Reacts with: Human
  • Isotype: IgG2a

Overview

  • Product name

    Anti-C5b-9 + C5b-8 antibody [aE11]

  • Description

    Mouse monoclonal [aE11] to C5b-9 + C5b-8

  • Host species

    Mouse

  • Specificity

    The aE11 monoclonal was first reported to bind to a neoepitope exposed on polymerized C9 when incorporated into the human terminal complement complex (PubMed ID: 4035298). It was subsequently shown that this antibody also cross reacts with the C5b-8 complex and purified C8 alpha-gamma. It does not react with native C9 or C8 proteins (PubMed ID: 2424021).

    ab66768 binds both membrane-bound MAC (active) and fluid-phase SC5b-9 complexes (inactive) (Mollnes TE, Harboe M (1987).

  • Tested applications

    Suitable for: IHC-Frmore details
    Unsuitable for: WB

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Full length protein corresponding to Human C5b-9.

  • Positive control

    • IHC-Fr: Human tonsil, monkey retina cord, and porcine liver tissues.

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

Images

  • Immunohistochemistry (Frozen sections) - Anti-C5b-9 + C5b-8 antibody [aE11] (ab66768)
    Immunohistochemistry (Frozen sections) - Anti-C5b-9 + C5b-8 antibody [aE11] (ab66768) Image from Ekser B et al., PLoS One. 2012;7(1):e29720. Epub 2012 Jan 11. Fig 3.; doi:10.1371/journal.pone.0029720; January 11, 2012, PLoS ONE 7(1): e29720.

    ab66768 staining C5b-9 + C5b-8 (red) in porcine liver tissue by Immunohistochemistry (Frozen sections).

    Tissue was taken from allotransplants of wild-type or a1,3-galactosyltransferase gene-knockout (GTKO) pig liver, 2 hours after grafting or at necroscopy (nec). Tissue was fixed with paraformaldehyde and blocked with 20% donkey serum for 1 hour at room temperature, before being incubated with primary antibody (1/100 in diluent).

  • Immunohistochemistry (Frozen sections) - Anti-C5b-9 + C5b-8 antibody [aE11] (ab66768)
    Immunohistochemistry (Frozen sections) - Anti-C5b-9 + C5b-8 antibody [aE11] (ab66768) This image is courtesy of an anonymous Abreview

    ab66768 staining C5b-9 + C5b-8 in monkey retina cord tissue sections by Immunohistochemistry (IHC-Fr - frozen sections).

    Tissue was fixed with paraformaldehyde, permeabilized with 0.5% Triton-X and blocked with 4% serum for 30 minutes at 22°C. Samples were incubated with primary antibody (1/500) for 16 hours at 4°C. An Alexa Fluor® 488-conjugated goat anti-mouse IgG polyclonal (1/300) was used as the secondary antibody. Counterstained with DAPI. Magnification: 20X.

    See Abreview

  • Immunohistochemistry (Frozen sections) - Anti-C5b-9 + C5b-8 antibody [aE11] (ab66768)
    Immunohistochemistry (Frozen sections) - Anti-C5b-9 + C5b-8 antibody [aE11] (ab66768)

    Immunohistochemical analysis of frozen tonsil tissue section labeling C5b-9 + C5b-8 with ab66768 at a dilution of 1/10.

    Vascular endothelia and dendritic cells of germinal center have strong and intensive label.

     

     

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