Human Complement C2 ELISA Kit (ab254501)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 175.11 pg/ml
- Range: 281.25 pg/ml - 18000 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Milk, Saliva, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Complement C2 ELISA Kit
See all C2 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Plasma 5 6% Inter-assay Sample n Mean SD CV% Plasma 3 5.5% -
Sample type
Cell culture supernatant, Saliva, Milk, Serum, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
175.11 pg/ml -
Range
281.25 pg/ml - 18000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 111 107% - 116% Saliva 115 113% - 116% Milk 107 104% - 114% Serum 100 94% - 107% Cell culture media 100 94% - 108% Hep Plasma 99 96% - 102% EDTA Plasma 106 96% - 121% Cit plasma 106 101% - 108% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human
Does not react with: Cow -
Product overview
Human Complement C2 ELISA Kit (ab254501) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Complement C2 protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, milk, saliva, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Complement C2 with 175.11 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Complement C2 is a serine protease involved in both the classical and lectin pathways of complement activation. C2 is secreted as a zymogen. Activation of C2 by C1 leads to the proteolysis of C2 into C2a and C2b. Deficiency of C2 can lead to various types of autoimmune diseases.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human Complement C2 Capture Antibody 1 x 600µl 10X Human Complement C2 Detector Antibody 2 vials 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent CPI - HAMA Blocker (ab193969) 1 x 6ml Human Complement C2 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Component C2 which is part of the classical pathway of the complement system is cleaved by activated factor C1 into two fragments: C2b and C2a. C2a, a serine protease, then combines with complement factor 4b to generate the C3 or C5 convertase. -
Involvement in disease
Defects in C2 are the cause of complement component 2 deficiency (C2D) [MIM:217000]. A deficiency of the complement classical pathway associated with the development of autoimmune disorders, mainly systemic lupus erythematosus. Skin and joint manifestations are common and renal disease is relatively rare. Patients with complement component 2 deficiency are also reported to have recurrent or invasive infections. -
Sequence similarities
Belongs to the peptidase S1 family.
Contains 1 peptidase S1 domain.
Contains 3 Sushi (CCP/SCR) domains.
Contains 1 VWFA domain. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- ARMD14
- C2
- C3/C5 convertase
see all -
Database links
- Entrez Gene: 717 Human
- Omim: 217000 Human
- SwissProt: P06681 Human
- Unigene: 408903 Human
Images
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SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of human Complement C2 standard curve in Sample Diluent NS.The Complement C2 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native Complement C2 in human serum and plasma samples.The concentrations of Complement C2 were measured in duplicates, interpolated from the Complement C2 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 1: 4,000, plasma (citrate) 1: 2,000, plasma (heparin) 1: 1,000, and plasma (EDTA) 1: 1,000. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Complement C2 concentration was determined to be 27.00 mg/mL in serum, 15.46 mg/mL in plasma (citrate), 11.89 mg/ml in plasma (heparin), and 12.51 mg/ml in plasma (EDTA).
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Interpolated concentrations of native Complement C2 in human cell culture supernatant, milk, and female saliva samples.The concentrations of Complement C2 were measured in duplicates, interpolated from the Complement C2 standard curves and corrected for sample dilution. Undiluted samples are as follows: HepG2 cell culture supernatant 1: 800, milk 12.5%, and female saliva 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Complement C2 concentration was determined to be 117.47 ng/mL in HepG2 cell culture supernatant, 117.91 ng/mL in milk, and 22.87 ng/mL in female saliva.
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Serum from ten individual healthy human female donors was measured in duplicate.Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Complement C2 concentration was determined to be 19.67 mg/mL with a range of 12.10 – 27.26 mg/mL.
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Sandwich ELISA - Human Complement C2 ELISA Kit (ab254501)To learn more about the advantages of recombinant antibodies see here.
