Antibodies, Proteins, Kits and Reagents for Life Science

324 988 ₸ Product size

100 µg 324 988 ₸ 10 µg 107 212 ₸

Order now and get it on Tuesday March 02, 2021

Anti-Sumo 2 + Sumo 3 antibody [8A2] (ab81371)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Mouse monoclonal [8A2] to Sumo 2 + Sumo 3
Suitable for: WB, ICC/IF, IHC-P
Reacts with: Mouse, Rat, Human

Product name

Anti-Sumo 2 + Sumo 3 antibody [8A2]
See all Sumo 2 + Sumo 3 primary antibodies
Description

Mouse monoclonal [8A2] to Sumo 2 + Sumo 3
Host species

Mouse
Specificity

This antibody recognizes Sumo 2 and 3 but does not recognize Sumo 1.

Tested Applications & Species

Application	Species
ICC/IF	Mouse Human
IHC-P	Mouse Rat Human
WB	Mouse Rat Human

See all applications and species data

Immunogen

Recombinant full length protein corresponding to Human Sumo 2 + Sumo 3.
Positive control
- WB: HeLa, SH-SY5Y, K562, SW480, Jurkat, HEK-293T, PC-12 and NIH/3T3 whole cell lysate. Human brain, spleen and kidney tissue lysate. Rat spleen tissue lysate. Mouse brain tissue lysate. IHC-P: Mouse and rat kidney tissue. Human breast tissue. ICC: HeLa, SW480 and NIH/3T3 cells.
General notes
This product has switched from ascites to recombinant production method on 25^th August 2020.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

8A2
Isotype

IgG2b
Research areas

Western blot - Anti-Sumo 2 + Sumo 3 antibody [8A2] - ChIP Grade (ab81371)

All lanes : Anti-Sumo 2 + Sumo 3 antibody [8A2] (ab81371) at 1/1000 dilution

Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate B276
Lane 2 : SH-SY5Y (human neuroblastoma epithelial cell), whole cell lysate
Lane 3 : K562 (human chronic myelogenous leukemia lymphoblast), whole cell lysate
Lane 4 : SW480 (human colorectal adenocarcinoma epithelial cell), whole cell lysate
Lane 5 : Jurkat (human T cell leukemia T lymphocyte), whole cell lysate
Lane 6 : 293T (human embryonic kidney epithelial cell), whole cell lysate
Lane 7 : PC-12 (rat adrenal gland pheochromocytoma), whole cell lysate
Lane 8 : NIH/3T3 (mouse embryonic fibroblast), whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L)

Predicted band size: 12 kDa
Observed band size: 15 kDa
why is the actual band size different from the predicted?

Buffer and concentration: 5% NFDM/TBST
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sumo 2 + Sumo 3 antibody [8A2] - ChIP Grade (ab81371)

Immunohistochemical analysis of paraffin-embedded rat kidney tissue labeling Sumo 2 + Sumo 3 with ab81371 at 1/1000 dilution followed by a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879). The section was incubated with ab81371 for overnight at 4℃.
Secondary antibody only control: Ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879).
Heat mediated antigen retrieval with citrate buffer (pH 6.0, epitope retrieval solution1).
Western blot - Anti-Sumo 2 + Sumo 3 antibody [8A2] - ChIP Grade (ab81371)

All lanes : Anti-Sumo 2 + Sumo 3 antibody [8A2] (ab81371) at 1/1000 dilution

Lane 1 : Human brain tissue lysate
Lane 2 : Human kidney tissue lysate
Lane 3 : Human spleen tissue lysate
Lane 4 : Mouse brain tissue lysate
Lane 5 : Rat spleen tissue lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

Predicted band size: 12 kDa
Observed band size: 15 kDa why is the actual band size different from the predicted?

Dilution and concentration: 5% NFDM/TBST
Immunocytochemistry/ Immunofluorescence - Anti-Sumo 2 + Sumo 3 antibody [8A2] - ChIP Grade (ab81371)

Immunocytochemistry/immunofluorescence analysis of NIH/3T3 cells labelling Sumo 2 + Sumo 3 with ab81371 at 1/50 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Primary antibody, ab81371 at 1:50 was incubated overnight at 4° C, followed by AlexaFluor® 488-conjugated Goat anti- Mouse secondary antibody (ab150113) at 1/1000 dilution at RT for 45 min. ab179513 Anti-beta Tubulin, used as a counterstain at 1/200 dilution, was co-incubated with ab81371 overnight at 4° C, followed by Alexa Fluor® 594 Goat Anti-Rabbit. secondary (ab150080) at 1/1000 dilution at RT for 45 min. Nucleus were visualized using DAPI.
Immunocytochemistry/ Immunofluorescence - Anti-Sumo 2 + Sumo 3 antibody [8A2] - ChIP Grade (ab81371)

Immunocytochemistry/immunofluorescence analysis of HeLa cells labelling Sumo 2 + Sumo 3 with ab81371 at 1/50 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Primary antibody, ab81371 at 1:50 was incubated overnight at 4° C, followed by AlexaFluor® 488-conjugated Goat anti- Mouse secondary antibody (ab150113) at 1/1000 dilution at RT for 45 min. ab179513 Anti-beta Tubulin, used as a counterstain at 1/200 dilution, was co-incubated with ab81371 overnight at 4° C, followed by Alexa Fluor® 594 Goat Anti-Rabbit. secondary (ab150080) at 1/1000 dilution at RT for 45 min. Nucleus were visualized using DAPI.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sumo 2 + Sumo 3 antibody [8A2] - ChIP Grade (ab81371)

Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling Sumo 2 + Sumo 3 with ab81371 at 1/1000 dilution followed by a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879). The section was incubated with ab81371 for overnight at 4℃.
Secondary antibody only control: Ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879).
Heat mediated antigen retrieval with citrate buffer (pH 6.0, epitope retrieval solution1).
Immunocytochemistry/ Immunofluorescence - Anti-Sumo 2 + Sumo 3 antibody [8A2] - ChIP Grade (ab81371)

Immunocytochemistry/immunofluorescence analysis of SW480 cell line labelling Sumo 2 + Sumo 3 with ab81371 at 1/50 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Primary antibody, ab81371 at 1:50 was incubated overnight at 4° C, followed by AlexaFluor® 488-conjugated Goat anti- Mouse secondary antibody (ab150113) at 1/1000 dilution at RT for 45 min. ab179513 Anti-beta Tubulin, used as a counterstain at 1/200 dilution, was co-incubated with ab81371 overnight at 4° C, followed by Alexa Fluor® 594 Goat Anti-Rabbit. secondary (ab150080) at 1/1000 dilution at RT for 45 min. Nucleus were visualized using DAPI.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sumo 2 + Sumo 3 antibody [8A2] - ChIP Grade (ab81371)

Immunohistochemical analysis of paraffin-embedded human breast tissue labeling Sumo 2 + Sumo 3 with ab81371 at 1/1000 dilution followed by a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879). The section was incubated with ab81371 for overnight at 4℃.
Secondary antibody only control: Ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879).
Heat mediated antigen retrieval with citrate buffer (pH 6.0, epitope retrieval solution1).
Anti-Sumo 2 + Sumo 3 antibody [8A2] (ab81371)

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