Anti-Rb (phospho S795) antibody (ab131347)
Key features and details
- Rabbit polyclonal to Rb (phospho S795)
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Rb (phospho S795) antibody
See all Rb primary antibodies -
Description
Rabbit polyclonal to Rb (phospho S795) -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide from around the phosphorylation site of serine 795 (
P-S-S(p)-P-L
) derived from Human Rb. (P06400). -
Positive control
- methanol fixed HeLa cells, Serum treated K562 cells, Human breast carcinoma tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Antibodies where purified using epitope specific phospho peptide. Non-phospho specific antibodies were removed by chromatography using non-phospho peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Rb (phospho S795) antibody (ab131347)All lanes : Anti-Rb (phospho S795) antibody (ab131347) at 1/500 dilution
Lane 1 : untreated K562 cell extracts
Lane 2 : Serum treated K562 cell extracts
Predicted band size: 106 kDa
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Immunocytochemistry/ Immunofluorescence - Anti-Rb (phospho S795) antibody (ab131347)Immunofluorescence staining of methanol fixed HeLa cells staining Rb (phospho Ser795) with ab131347 antibody at a dilution of 1/100. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rb (phospho S795) antibody (ab131347)Immunohistochemical staining of human breast carcinoma tissue labelling Rb (phospho Ser795) with ab131347 at 1/50 dilution (left) or following antibody preincubation with immunizing peptide (right).
