Antibodies, Proteins, Kits and Reagents for Life Science

294 835 ₸ Product size

100 µl 294 835 ₸

Order now and get it on Tuesday March 02, 2021

Anti-Rb (phospho S807) antibody [EPR17732] (ab184796)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR17732] to Rb (phospho S807)
Suitable for: Dot blot, WB, ICC/IF, IHC-P
Reacts with: Mouse, Rat, Human

Product name

Anti-Rb (phospho S807) antibody [EPR17732]
See all Rb primary antibodies
Description

Rabbit monoclonal [EPR17732] to Rb (phospho S807)
Host species

Rabbit

Tested Applications & Species

Application	Species
ICC/IF	Human
IHC-P	Mouse Rat Human
WB	Mouse Rat Human

See all applications and species data

Immunogen

This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: K562, MCF7, L-929 and C6 whole cell lysates; Mouse embryo lysate. IHC-P: Human colon, Human squamous cells carcinoma of lung, mouse spleen and rat spleen tissues. ICC/IF: MCF7 cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR17732
Isotype

IgG
Research areas

Western blot - Anti-Rb (phospho S807) antibody [EPR17732] (ab184796)

All lanes : Anti-Rb (phospho S807) antibody [EPR17732] (ab184796) at 1/20000 dilution

Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) serum starved for 44 h. Whole cell lysates
Lane 2 : Jurkat (human T cell leukemia cell line from peripheral blood) serum starved for 24 h. Then 10% FBS incubated for 20 h. Whole cell lysates
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) serum starved for 24 h. Then 10% FBS incubated for 20 h. Whole cell lysates. Then the membrane was incubated with phosphatase.

Lysates/proteins at 15 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 106 kDa
Observed band size: 106 kDa

Exposure time: 10 seconds

Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Immunocytochemistry/ Immunofluorescence - Anti-Rb (phospho S807) antibody [EPR17732] (ab184796)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% tritonX-100 permeabilized MCF7 (human breast adenocarcinoma cell line) cells, Serum starved and non-starved, labeling Rb (phospho S807) with Ab184796 at 1/200 dilution followed by Goat anti-Rabbit secondary IgG AlexaFluor®488 (ab150077) secondary antibody at 1/1000 dilution (green).

Confocal image showing positive staining on MCF7 cells. The number of positive cells increased after treatment with FBS (fetal bovine serum) for 48 hours, then decreased after Lambda Protein Phosphatase treatment (31℃ for 2 hours ).
Western blot - Anti-Rb (phospho S807) antibody [EPR17732] (ab184796)

All lanes : Anti-Rb (phospho S807) antibody [EPR17732] (ab184796) at 1/20000 dilution

Lane 1 : K562 (human chronic myelogenous leukemia cells from bone marrow) whole cell lysates
Lane 2 : K562 (human chronic myelogenous leukemia cells from bone marrow) whole cell lysates serum starved for 2 days

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Predicted band size: 106 kDa
Observed band size: 106 kDa

Exposure time: 3 minutes

Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot - Anti-Rb (phospho S807) antibody [EPR17732] (ab184796)

All lanes : Anti-Rb (phospho S807) antibody [EPR17732] (ab184796) at 1/5000 dilution

Lane 1 : K562 (human chronic myelogenous leukemia cells from bone marrow) whole cell lysates
Lane 2 : K562 (human chronic myelogenous leukemia cells from bone marrow) whole cell lysates treated with Lambda Phosphatase.

Lysates/proteins at 10 µg per lane.

Secondary
Lane 1 : Goat Anti-Rabbit IgG (H+L), Peroxidase conjugated at 1/1000 dilution
Lane 2 : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Predicted band size: 106 kDa
Observed band size: 106 kDa

Exposure time: 30 seconds

Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot - Anti-Rb (phospho S807) antibody [EPR17732] (ab184796)

Anti-Rb (phospho S807) antibody [EPR17732] (ab184796) at 1/1000 dilution + L-929 (mouse connective tissue fibroblast cell line) whole cell lysate at 10 µg

Secondary
Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Predicted band size: 106 kDa
Observed band size: 106 kDa

Exposure time: 3 minutes

Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot - Anti-Rb (phospho S807) antibody [EPR17732] (ab184796)

Anti-Rb (phospho S807) antibody [EPR17732] (ab184796) at 1/2000 dilution + Mouse embryo lysate at 10 µg

Secondary
Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Predicted band size: 106 kDa
Observed band size: 106 kDa

Exposure time: 3 minutes

Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot - Anti-Rb (phospho S807) antibody [EPR17732] (ab184796)

Anti-Rb (phospho S807) antibody [EPR17732] (ab184796) at 1/2000 dilution + C6 (rat glial tumor cell line) whole cell lysate at 10 µg

Secondary
Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Predicted band size: 106 kDa
Observed band size: 106 kDa

Exposure time: 30 seconds

Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot - Anti-Rb (phospho S807) antibody [EPR17732] (ab184796)

Anti-Rb (phospho S807) antibody [EPR17732] (ab184796) at 1/1000 dilution + MCF7 (human breast adenocarcinoma cell line) whole cell lysate at 10 µg

Secondary
Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Predicted band size: 106 kDa
Observed band size: 106 kDa

Exposure time: 3 minutes

Blocking/Dilution buffer: 5% NFDM/TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rb (phospho S807) antibody [EPR17732] (ab184796)

Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Rb (phospho S807) with ab184796 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Nucleus staining on epithelial cells of Human colon is observed. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rb (phospho S807) antibody [EPR17732] (ab184796)

Immunohistochemical analysis of paraffin-embedded human Squamous cells carcinoma of lung tissue labeling Rb (phospho S807) with ab184796 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Nucleus staining on squamous cells carcinoma of lung is observed. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rb (phospho S807) antibody [EPR17732] (ab184796)

Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling Rb (phospho S807) with ab184796 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Nucleus staining on lymphocytes of mouse spleen is observed. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rb (phospho S807) antibody [EPR17732] (ab184796)

Immunohistochemical analysis of paraffin-embedded rat spleen tissue labeling Rb (phospho S807) with ab184796 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Nucleus staining on lymphocytes of rat spleen is observed. Counter stained with Hematoxylin.

Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Dot Blot - Anti-Rb (phospho S807) antibody [EPR17732] (ab184796)

Dot blot analysis of Rb (phospho S807) peptide (Lane 1), and non-phospho peptide (Lane 2), labeled using ab184796 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated secondary antibody at 1/1000 dilution.
Blocking/Dilution buffer: 5% NFDM/TBST.
Anti-Rb (phospho S807) antibody [EPR17732] (ab184796)

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