Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Rb antibody [E182] - BSA and Azide free (ab239820)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [E182] to Rb - BSA and Azide free
  • Suitable for: IP, ICC/IF, Flow Cyt, WB
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-Rb antibody [E182] - BSA and Azide free
    See all Rb primary antibodies

  • Description

    Rabbit monoclonal [E182] to Rb - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IP, ICC/IF, Flow Cyt, WBmore details
    Unsuitable for: IHC

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Hek293, and Jurkat cell lysates; ICC: A431 cells; Flow cyt: Jurkat cells.

  • General notes

    ab239820 is the carrier-free version of ab32513 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab239820 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Immunoprecipitation - Anti-Rb antibody [E182] - BSA and Azide free (ab239820)
    Immunoprecipitation - Anti-Rb antibody [E182] - BSA and Azide free (ab239820)

    This data was developed using ab32513, the same antibody clone in a different buffer formulation.
    Purified ab32513 at 1/40 dilution (2µg) immunoprecipitating Rb in Jurkat whole cell lysate.
    Lane 1 (input): Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate 10µg
    Lane 2 (+): ab32513 + Jurkat whole cell lysate.
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab32513 in Jurkat whole cell lysate.
    VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
    Blocking Buffer and concentration: 5% NFDM/TBST.
    Diluting buffer and concentration: 5% NFDM/TBST.
    Observed band size: 120 kDa

  • Western blot - Anti-Rb antibody [E182] - BSA and Azide free (ab239820)
    Western blot - Anti-Rb antibody [E182] - BSA and Azide free (ab239820)
    All lanes : Anti-Rb antibody [E182] (ab32513) at 1/1000 dilution (Purified)

    Lane 1 : Jurkat (Human T cell leukemia T lymphocyte) prepared in RIPA lysis method whole cell lysate
    Lane 2 : Jurkat (Human T cell leukemia T lymphocyte) prepared in 1% SDS Hot lysis method whole cell lysate

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 106 kDa
    Observed band size: 106-120 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab32513, the same antibody clone in a different buffer formulation.

    1% SDS Hot lysis method is preferred for this antibody.

    Blocking Buffer and concentration: 5% NFDM/TBST

  • Immunocytochemistry - Anti-Rb antibody [E182] - BSA and Azide free (ab239820)
    Immunocytochemistry - Anti-Rb antibody [E182] - BSA and Azide free (ab239820)

    This data was developed using ab32513, the same antibody clone in a different buffer formulation.
    Immunocytochemistry analysis of A431 (Human epidermoid carcinoma epithelial cell) cells labeling Rb with Purified ab239820 at 1:50 dilution (10 ?g/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Flow Cytometry - Anti-Rb antibody [E182] - BSA and Azide free (ab239820)
    Flow Cytometry - Anti-Rb antibody [E182] - BSA and Azide free (ab239820)

    This data was developed using ab32513, the same antibody clone in a different buffer formulation.
    Flow Cytometry analysis of Jurkat (Human T cell leukemia T lymphocyte) cells labeling Rb with Purified ab239820 at 1:70 dilution (10 µg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488,ab150077) secondary antibody was used at 1:2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

  • Anti-Rb antibody [E182] - BSA and Azide free (ab239820)
    Anti-Rb antibody [E182] - BSA and Azide free (ab239820)

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