Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Rb (phospho T356) antibody [EPR2153AY] (ab76298)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2153AY] to Rb (phospho T356)
  • Suitable for: WB, IP, IHC-P, Dot blot
  • Reacts with: Human

Overview

  • Product name

    Anti-Rb (phospho T356) antibody [EPR2153AY]
    See all Rb primary antibodies

  • Description

    Rabbit monoclonal [EPR2153AY] to Rb (phospho T356)

  • Host species

    Rabbit

  • Specificity

    The antibody only detects Rb phosphorylated on Threonine 356.

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Jurkat cell lysate treated with FBS Human breast carcinoma tissue HeLa cells IP: Jurkat whole cell lysate.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rb (phospho T356) antibody [EPR2153AY] (ab76298)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rb (phospho T356) antibody [EPR2153AY] (ab76298)

    ab76298 showing positive staining in Endometrial carcinoma tissue.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Western blot - Anti-Rb (phospho T356) antibody [EPR2153AY] (ab76298)
    Western blot - Anti-Rb (phospho T356) antibody [EPR2153AY] (ab76298)
    All lanes : Anti-Rb (phospho T356) antibody [EPR2153AY] (ab76298) at 1/4000 dilution

    Lane 1 : K562 (Human chronic myelogenous leukemia cell line from bone marrow) 2 day serum starved whole cell lysate.
    Lane 2 : K562 (Human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate.
    Lane 3 : K562 (Human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate. Membrane incubated with phosphatase.

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 106 kDa
    Observed band size: 106 kDa


    Exposure time: 1 minute
  • Immunoprecipitation - Anti-Rb (phospho T356) antibody [EPR2153AY] (ab76298)
    Immunoprecipitation - Anti-Rb (phospho T356) antibody [EPR2153AY] (ab76298)

    Purified ab76298 at 1/20 dilution (0.5µg) immunoprecipitating Rb in Jurkat whole cell lysate.
    Lane 1 (input): Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate 10µg
    Lane 2 (+): ab76298 + Jurkat whole cell lysate.
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab76298 in Jurkat whole cell lysate.
    VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
    Blocking Buffer and concentration: 5% NFDM/TBST.
    Diluting buffer and concentration: 5% NFDM/TBST.
    Observed band size: 110 kDa
    Cleavage fragments were observed as described in literature. (PMID: 11420704)

  • Dot Blot - Anti-Rb (phospho T356) antibody [EPR2153AY] (ab76298)
    Dot Blot - Anti-Rb (phospho T356) antibody [EPR2153AY] (ab76298)

    Dot blot analysis of Rb (phospho T356) phospho peptide (Lane 1), Rb Non-phospho peptide (Lane 2), labelling Rb (phospho T356) with ab76298 at a dilution of 1/1000. Peroxidase conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody at a dilution of 1/2500.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: 3 minutes.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rb (phospho T356) antibody [EPR2153AY] (ab76298)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rb (phospho T356) antibody [EPR2153AY] (ab76298)

    ab76298 showing positive staining in Lung adenocarcinoma tissue.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rb (phospho T356) antibody [EPR2153AY] (ab76298)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rb (phospho T356) antibody [EPR2153AY] (ab76298)

    ab76298 showing positive staining in Ovarian carcinoma tissue.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rb (phospho T356) antibody [EPR2153AY] (ab76298)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rb (phospho T356) antibody [EPR2153AY] (ab76298)

    ab76298 showing positive staining in Normal tonsil tissue.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rb (phospho T356) antibody [EPR2153AY] (ab76298)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rb (phospho T356) antibody [EPR2153AY] (ab76298)

    Immunohistochemical analysis of paraffin-embedded human breast carcinoma using ab76298 at 1/100-1/250 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Western blot - Anti-Rb (phospho T356) antibody [EPR2153AY] (ab76298)
    Western blot - Anti-Rb (phospho T356) antibody [EPR2153AY] (ab76298)
    All lanes : Anti-Rb (phospho T356) antibody [EPR2153AY] (ab76298) at 1/2000 dilution

    Lane 1 : Jurkat cell lysate
    Lane 2 : Jurkat cell lysate treated with FBS

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 106 kDa
    Observed band size: 106 kDa

  • Anti-Rb (phospho T356) antibody [EPR2153AY] (ab76298)
    Anti-Rb (phospho T356) antibody [EPR2153AY] (ab76298)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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