Anti-BACH2 antibody [7A4] - BSA and Azide free (ab252254)
![Anti-BACH2 antibody [7A4] - BSA and Azide free (ab252254)](https://www.abcam.com/ps/products/252/ab252254/Images/ab252254-367721-anti-bach2-antibody-western-blot-hek293t-lysate.jpg "Anti-BACH2 antibody [7A4] - BSA and Azide free (ab252254)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rat monoclonal [7A4] to BACH2 - BSA and Azide free
- Suitable for: WB
- Reacts with: Mouse, Human
Overview
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Product name
Anti-BACH2 antibody [7A4] - BSA and Azide free
See all BACH2 primary antibodies -
Description
Rat monoclonal [7A4] to BACH2 - BSA and Azide free -
Host species
Rat -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HEK-293T, Ramos, NAMALWA and WEHI-231 whole cell lysate.
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
ab252254 is the carrier-free version of ab243148. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Ion Exchange Chromatography -
Clonality
Monoclonal -
Clone number
7A4 -
Isotype
IgG2a -
Research areas
Images
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Western blot - Anti-BACH2 antibody [7A4] - BSA and Azide free (ab252254)All lanes : Anti-BACH2 antibody [7A4] (ab243148) at 0.502 µg/ml
Lane 1 : 293T (human embryonic kidney epithelial cell), whole cell lysate,
Lane 2 : Ramos (human Burkitt's lymphoma B lymphocyte), whole cell lysate
Lane 3 : THP-1 (human monocytic leukemia monocyte), whole cell lysate
Lane 4 : HL-60 (human Acute Promyelocytic Leukemia promyeloblast), whole cell lysate
Lane 5 : NAMALWA (human Burkitt's lymphoma B lymphocyte), whole cell lysate
Lane 6 : WEHI-231 (mouse B cell lymphoma B lymphocyte), whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/5000 dilution
Predicted band size: 93 kDaThis data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab243148).
Blocking/diluting buffer and concentration: 5% NFDM/TBST.
Exposure time:
Lane 1: 3 minutes
Lanes 2-5: 3 seconds
Lane 6: 5 secondsNegative control: THP-1 and HL-60 (PMID: 10949928).
The molecular weight observed is consistent with what has been described in the literature (PMID: 9755173).
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![Anti-BACH2 antibody [7A4] - BSA and Azide free (ab252254)](https://www.abcam.com/ps/products/252/ab252254/Images/ab252254-2-benefits-of-recombinant-antibodies.png)
Anti-BACH2 antibody [7A4] - BSA and Azide free (ab252254)
