Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-BACH2 antibody [7A4] - BSA and Azide free (ab252254)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rat monoclonal [7A4] to BACH2 - BSA and Azide free
  • Suitable for: WB
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-BACH2 antibody [7A4] - BSA and Azide free
    See all BACH2 primary antibodies

  • Description

    Rat monoclonal [7A4] to BACH2 - BSA and Azide free

  • Host species

    Rat

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HEK-293T, Ramos, NAMALWA and WEHI-231 whole cell lysate.

  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    ab252254 is the carrier-free version of ab243148. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

Images

  • Western blot - Anti-BACH2 antibody [7A4] - BSA and Azide free (ab252254)
    Western blot - Anti-BACH2 antibody [7A4] - BSA and Azide free (ab252254)
    All lanes : Anti-BACH2 antibody [7A4] (ab243148) at 0.502 µg/ml

    Lane 1 : 293T (human embryonic kidney epithelial cell), whole cell lysate,
    Lane 2 : Ramos (human Burkitt's lymphoma B lymphocyte), whole cell lysate
    Lane 3 : THP-1 (human monocytic leukemia monocyte), whole cell lysate
    Lane 4 : HL-60 (human Acute Promyelocytic Leukemia promyeloblast), whole cell lysate
    Lane 5 : NAMALWA (human Burkitt's lymphoma B lymphocyte), whole cell lysate
    Lane 6 : WEHI-231 (mouse B cell lymphoma B lymphocyte), whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/5000 dilution

    Predicted band size: 93 kDa



    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab243148).

    Blocking/diluting buffer and concentration: 5% NFDM/TBST.

    Exposure time:

    Lane 1: 3 minutes 
    Lanes 2-5: 3 seconds
    Lane 6: 5 seconds

    Negative control: THP-1 and HL-60 (PMID: 10949928).

    The molecular weight observed is consistent with what has been described in the literature (PMID: 9755173).

     

  • Anti-BACH2 antibody [7A4] - BSA and Azide free (ab252254)
    Anti-BACH2 antibody [7A4] - BSA and Azide free (ab252254)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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