Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-KLHL6 antibody [EPR13484] (ab182163)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13484] to KLHL6
  • Suitable for: WB, ICC/IF, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-KLHL6 antibody [EPR13484]
    See all KLHL6 primary antibodies

  • Description

    Rabbit monoclonal [EPR13484] to KLHL6

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Human fetal kidney and A549 lysates; A549 cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR13484

  • Isotype

    IgG

  • Research areas

Images

  • Immunoprecipitation - Anti-KLHL6 antibody [EPR13484] (ab182163)
    Immunoprecipitation - Anti-KLHL6 antibody [EPR13484] (ab182163)

    Western blot analysis of immunoprecipitation pellet from A549 cell lysate immunoprecipitated using ab182163 at 1/50 dilution.

    Secondary: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-KLHL6 antibody [EPR13484] (ab182163)
    Immunocytochemistry/ Immunofluorescence - Anti-KLHL6 antibody [EPR13484] (ab182163)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed A549 cells labeling KLHL6 with ab182163 at 1/250 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 488) secondary at 1/200 dilution. Counter stained with DAPI.

  • Western blot - Anti-KLHL6 antibody [EPR13484] (ab182163)
    Western blot - Anti-KLHL6 antibody [EPR13484] (ab182163)
    Anti-KLHL6 antibody [EPR13484] (ab182163) at 1/1000 dilution + A549 cell lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 70 kDa
    Observed band size: 70 kDa

  • Western blot - Anti-KLHL6 antibody [EPR13484] (ab182163)
    Western blot - Anti-KLHL6 antibody [EPR13484] (ab182163)
    Anti-KLHL6 antibody [EPR13484] (ab182163) at 1/1000 dilution + Human fetal kidney lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab136636) at 1/500 dilution

    Predicted band size: 70 kDa
    Observed band size: 70 kDa

  • Anti-KLHL6 antibody [EPR13484] (ab182163)
    Anti-KLHL6 antibody [EPR13484] (ab182163)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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