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Anti-Calcineurin A antibody [EPR1670(2)] - BSA and Azide free (ab239975)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR1670(2)] to Calcineurin A - BSA and Azide free
  • Suitable for: Flow Cyt, ICC/IF, WB
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Calcineurin A antibody [EPR1670(2)] - BSA and Azide free
    See all Calcineurin A primary antibodies

  • Description

    Rabbit monoclonal [EPR1670(2)] to Calcineurin A - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt, ICC/IF, WBmore details
    Unsuitable for: IHC-P or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa cell lysate; Mouse and rat brain cortex lysate Flow cyt: HeLa cells ICC/IF: HeLa cells

  • General notes

    Ab239975 is the carrier-free version of ab109412. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab239975 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Calcineurin A antibody [EPR1670(2)] - BSA and Azide free (ab239975)
    Western blot - Anti-Calcineurin A antibody [EPR1670(2)] - BSA and Azide free (ab239975)
    All lanes : Anti-Calcineurin A antibody [EPR1670(2)] (ab109412) at 1/10000 dilution

    Lane 1 : Wild-type HeLa cell lysate
    Lane 2 : PPP3CA knockout HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 59 kDa
    Observed band size: 59 kDa



    This data was developed using the same antibody clone in a different buffer formulation (ab109412).

    Lanes 1- 2: Merged signal (red and green). Green - ab109412 observed at 59 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (ab8245) observed at 37 kDa.

     ab109412 was shown to react with Calcineurin A in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab265130 (knockout cell lysate ab257181) was used. Wild-type HeLa and PPP3CA knockout HeLa cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab109412 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) overnight at 4°C at a 1 in 10000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Flow Cytometry - Anti-Calcineurin A antibody [EPR1670(2)] - BSA and Azide free (ab239975)
    Flow Cytometry - Anti-Calcineurin A antibody [EPR1670(2)] - BSA and Azide free (ab239975)
    Overlay histogram showing HeLa cells stained with ab109412 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab109412, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109412).

  • Immunocytochemistry/ Immunofluorescence - Anti-Calcineurin A antibody [EPR1670(2)] - BSA and Azide free (ab239975)
    Immunocytochemistry/ Immunofluorescence - Anti-Calcineurin A antibody [EPR1670(2)] - BSA and Azide free (ab239975)
    Immunofluorescent staining of HeLa cells using 1/100 ab109412.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109412).

  • Western blot - Anti-Calcineurin A antibody [EPR1670(2)] - BSA and Azide free (ab239975)
    Western blot - Anti-Calcineurin A antibody [EPR1670(2)] - BSA and Azide free (ab239975)
    All lanes : Anti-Calcineurin A antibody [EPR1670(2)] (ab109412) at 1/3000 dilution

    Lane 1 : Mouse brain cortex lysate
    Lane 2 : Mouse lung lysate
    Lane 3 : Rat brain cortex lysate
    Lane 4 : Rat liver lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/20000 dilution

    Predicted band size: 59 kDa
    Observed band size: 59 kDa



    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109412).

  • Anti-Calcineurin A antibody [EPR1670(2)] - BSA and Azide free (ab239975)
    Anti-Calcineurin A antibody [EPR1670(2)] - BSA and Azide free (ab239975)

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