Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Pepsinogen II/PGC antibody [EPR22843-269] - BSA and Azide free (ab259997)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22843-269] to Pepsinogen II/PGC - BSA and Azide free
  • Suitable for: WB, IHC-P, IHC-Fr
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Pepsinogen II/PGC antibody [EPR22843-269] - BSA and Azide free
    See all Pepsinogen II/PGC primary antibodies

  • Description

    Rabbit monoclonal [EPR22843-269] to Pepsinogen II/PGC - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, IHC-Frmore details
    Unsuitable for: IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: His-tagged Recombinant Human Pepsinogen C/PGC protein, Human stomach, Human testis, MDA-MB-231, Rat stomach and Mouse stomach lysates. IHC-P: Human stomach, Mouse stomach and Rat stomach tissues.

  • General notes

    Ab259997 is the carrier-free version of ab255826. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab259997 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pepsinogen II/PGC antibody [EPR22843-269] - BSA and Azide free (ab259997)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pepsinogen II/PGC antibody [EPR22843-269] - BSA and Azide free (ab259997)

    Immunohistochemical analysis of paraffin-embedded Rat stomach tissue labeling Pepsinogen II/PGC with ab255826 at 1/5000 dilution (0.129 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on rat stomach. The section was incubated with ab255826 for 10 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control/ Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab255826).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pepsinogen II/PGC antibody [EPR22843-269] - BSA and Azide free (ab259997)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pepsinogen II/PGC antibody [EPR22843-269] - BSA and Azide free (ab259997)

    Immunohistochemical analysis of paraffin-embedded Mouse stomach tissue labeling Pepsinogen II/PGC with ab255826 at 1/5000 dilution (0.129 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on mouse stomach. The section was incubated with ab255826 for 10 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control/ Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab255826).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pepsinogen II/PGC antibody [EPR22843-269] - BSA and Azide free (ab259997)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pepsinogen II/PGC antibody [EPR22843-269] - BSA and Azide free (ab259997)

    Immunohistochemical analysis of paraffin-embedded Human stomach tissue labeling Pepsinogen II/PGC with ab255826 at 1/5000 dilution (0.129 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human stomach (PMID 10883891). The section was incubated with ab255826 for 10 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control/ Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab255826).

  • Immunohistochemistry (Frozen sections) - Anti-Pepsinogen II/PGC antibody [EPR22843-269] - BSA and Azide free (ab259997)
    Immunohistochemistry (Frozen sections) - Anti-Pepsinogen II/PGC antibody [EPR22843-269] - BSA and Azide free (ab259997)

    Immunohistochemical analysis of frozen section of 4% PFA-fixed, 0.2% Triton X-100 permeabilized rat stomach tissue labeling Annexin-4/ANXA4 with ab255826 at 1/500 dilution, followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution (green). Positive staining on rat gastric mucosa (PMID: 10883891) is observed. The nuclear counter stain is DAPI (blue).
    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab255826).

  • Immunohistochemistry (Frozen sections) - Anti-Pepsinogen II/PGC antibody [EPR22843-269] - BSA and Azide free (ab259997)
    Immunohistochemistry (Frozen sections) - Anti-Pepsinogen II/PGC antibody [EPR22843-269] - BSA and Azide free (ab259997)

    Immunohistochemical analysis of frozen section of 4% PFA-fixed, 0.2% Triton X-100 permeabilized mouse stomach tissue labeling Annexin-4/ANXA4 with ab255826 at 1/500 dilution, followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution (green). Positive staining on mouse gastric mucosa (PMID: 10883891) is observed. The nuclear counter stain is DAPI (blue).
    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab255826).

  • Anti-Pepsinogen II/PGC antibody [EPR22843-269] - BSA and Azide free (ab259997)
    Anti-Pepsinogen II/PGC antibody [EPR22843-269] - BSA and Azide free (ab259997)

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