Antibodies, Proteins, Kits and Reagents for Life Science

298 185 ₸ Product size

100 µl 298 185 ₸

Order now and get it on Tuesday March 02, 2021

Anti-AGXT antibody [EPR13232] (ab178708)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR13232] to AGXT
Suitable for: WB, IP, IHC-P
Reacts with: Mouse, Human

Product name

Anti-AGXT antibody [EPR13232]
See all AGXT primary antibodies
Description

Rabbit monoclonal [EPR13232] to AGXT
Host species

Rabbit

Tested Applications & Species

Application	Species
IHC-P	Human
IP	Human
WB	Mouse Human

See all applications and species data

Immunogen

Recombinant fragment corresponding to Human AGXT.
Database link: P21549
Positive control
- HepG2 and Human fetal liver lysates; Human hepatocellular carcinoma and Human liver tissue.
General notes
Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Mouse species is recommended by WB application. We have preliminary internal testing data to indicate this antibody may not react with mouse in IHC.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR13232
Isotype

IgG
Research areas

Western blot - Anti-AGXT antibody [EPR13232] (ab178708)

All lanes : Anti-AGXT antibody [EPR13232] (ab178708) at 1/1000 dilution

Lane 1 : HepG2 cell lysate
Lane 2 : Human fetal liver lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 43 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AGXT antibody [EPR13232] (ab178708)

Immunohistochemical analysis of paraffin-embedded Human hepatocellular carcinoma tissue labeling AGXT with ab178708 at 1/100 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AGXT antibody [EPR13232] (ab178708)

Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling AGXT with ab178708 at 1/100 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunoprecipitation - Anti-AGXT antibody [EPR13232] (ab178708)

Western blot analysis on immunoprecipitation pellet from (1) Human fetal liver cell lysate or (2) 1X PBS (negative control) immunoprecipitated using ab170891 at 1/10 dilution. Detection utilized HRP-conjugated anti-rabbit IgG which preferentially detects the non-reduced form of rabbit IgG.
Western blot - Anti-AGXT antibody [EPR13232] (ab178708)

Western blot analysis of Mouse liver (20μg) labelling AGXT with ab178708 at 1/2000 in 5% NFDM /TBST. A Peroxidase conjugated Goat Anti-Rabbit IgG, (H+L), (ab97051) (1/20000) was used as the secondary antibody.
Anti-AGXT antibody [EPR13232] (ab178708)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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