Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-AGXT antibody [EPR22885-58] (ab261910)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22885-58] to AGXT
  • Suitable for: WB, IHC-P, IHC-Fr, Flow Cyt
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-AGXT antibody [EPR22885-58]
    See all AGXT primary antibodies

  • Description

    Rabbit monoclonal [EPR22885-58] to AGXT

  • Host species

    Rabbit

  • Specificity

    This antibody is not recommended for rat IHC-fr.

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Mouse
    IHC-Fr
    Mouse
    IHC-P
    Mouse
    Rat
    Human
    WB
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human liver, Mouse liver and Rat liver lysates. IHC-P: Human liver, Human liver cancer and Mouse liver tissues. IHC-Fr: Mouse liver tissue. Flow Cyt: Hepa1-6 cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-AGXT antibody [EPR22885-58] (ab261910)
    Western blot - Anti-AGXT antibody [EPR22885-58] (ab261910)
    All lanes : Anti-AGXT antibody [EPR22885-58] (ab261910) at 1/1000 dilution

    Lane 1 : Human liver tissue lysate
    Lane 2 : Mouse liver tissue lysate
    Lane 3 : Rat liver tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Lane 1 : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/250 dilution
    Lanes 2-3 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 43 kDa
    Observed band size: 43 kDa



    Blocking and diluting buffer and concentration: 5% NFDM/TBST The expression profile observed is consistent with what has been described in the literature (PMID: 16309382). 

    Exposure time: Lanes 1/2: 1 second; Lane 3: 59 seconds.

  • Flow Cytometry - Anti-AGXT antibody [EPR22885-58] (ab261910)
    Flow Cytometry - Anti-AGXT antibody [EPR22885-58] (ab261910)

    Flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized Hepa1-6 (mouse hepatoma epithelial cell) cells labelling AGXT with ab261910 at 1/50 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730) / Black isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AGXT antibody [EPR22885-58] (ab261910)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AGXT antibody [EPR22885-58] (ab261910)

    Immunohistochemical analysis of paraffin-embedded rat liver tissue labeling AGXT with ab261910 at 1/1000 dilution (0.502ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining in rat liver (PMID: 29883780) is observed. The section was incubated with ab261910 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AGXT antibody [EPR22885-58] (ab261910)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AGXT antibody [EPR22885-58] (ab261910)

    Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling AGXT with ab261910 at 1/ 1000 dilution (0.502ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining in mouse liver (PMID: 29883780) is observed. The section was incubated with ab261910 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AGXT antibody [EPR22885-58] (ab261910)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AGXT antibody [EPR22885-58] (ab261910)

    Immunohistochemical analysis of paraffin-embedded Human liver cancer tissue labeling AGXT with ab261910 at 1/ 1000 dilution (0.502ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining in human liver cancer (PMID: 29883780) is observed. The section was incubated with ab261910 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AGXT antibody [EPR22885-58] (ab261910)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AGXT antibody [EPR22885-58] (ab261910)

    Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling AGXT with ab261910 at 1/ 1000 dilution (0.502ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining in human liver (PMID: 29883780) is observed. The section was incubated with ab261910  for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

  • Immunohistochemistry (Frozen sections) - Anti-AGXT antibody [EPR22885-58] (ab261910)
    Immunohistochemistry (Frozen sections) - Anti-AGXT antibody [EPR22885-58] (ab261910)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen section of mouse liver tissue labeling AGXT with ab261910 at 1/100 dilution, followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution (green). The nuclear counter stain is DAPI (blue). Positive staining on mouse liver (PMID: 29883780).

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

  • Anti-AGXT antibody [EPR22885-58] (ab261910)
    Anti-AGXT antibody [EPR22885-58] (ab261910)

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