Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-AGXT antibody [EPR22885-58] - BSA and Azide free (ab261914)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22885-58] to AGXT - BSA and Azide free
  • Suitable for: WB, IHC-P, IHC-Fr, Flow Cyt
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-AGXT antibody [EPR22885-58] - BSA and Azide free
    See all AGXT primary antibodies

  • Description

    Rabbit monoclonal [EPR22885-58] to AGXT - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    This antibody is not recommended for rat IHC-fr.

  • Tested applications

    Suitable for: WB, IHC-P, IHC-Fr, Flow Cytmore details
    Unsuitable for: ICC/IF or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human liver, Mouse liver and Rat liver lysates. IHC-P: Human liver, Human liver cancer and Mouse liver tissues. IHC-Fr: Mouse liver tissue. Flow Cyt: Hepa1-6 cells.

  • General notes

    Ab261914 is the carrier-free version of ab261910. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab261914 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Flow Cytometry - Anti-AGXT antibody [EPR22885-58] - BSA and Azide free (ab261914)
    Flow Cytometry - Anti-AGXT antibody [EPR22885-58] - BSA and Azide free (ab261914)

    Flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized Hepa1-6 (mouse hepatoma epithelial cell) cells labelling AGXT with ab261910 at 1/50 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730) / Black isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab261910).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AGXT antibody [EPR22885-58] - BSA and Azide free (ab261914)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AGXT antibody [EPR22885-58] - BSA and Azide free (ab261914)

    Immunohistochemical analysis of paraffin-embedded rat liver tissue labeling AGXT with ab261910 at 1/1000 dilution (0.502ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining in rat liver (PMID: 29883780) is observed. The section was incubated with ab261910 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab261910).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AGXT antibody [EPR22885-58] - BSA and Azide free (ab261914)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AGXT antibody [EPR22885-58] - BSA and Azide free (ab261914)

    Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling AGXT with ab261910 at 1/ 1000 dilution (0.502ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining in mouse liver (PMID: 29883780) is observed. The section was incubated with ab261910 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab261910).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AGXT antibody [EPR22885-58] - BSA and Azide free (ab261914)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AGXT antibody [EPR22885-58] - BSA and Azide free (ab261914)

    Immunohistochemical analysis of paraffin-embedded Human liver cancer tissue labeling AGXT with ab261910 at 1/ 1000 dilution (0.502ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining in human liver cancer (PMID: 29883780) is observed. The section was incubated with ab261910 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab261910).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AGXT antibody [EPR22885-58] - BSA and Azide free (ab261914)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AGXT antibody [EPR22885-58] - BSA and Azide free (ab261914)

    Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling AGXT with ab261910 at 1/ 1000 dilution (0.502ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining in human liver (PMID: 29883780) is observed. The section was incubated with ab261910  for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab261910).

  • Immunohistochemistry (Frozen sections) - Anti-AGXT antibody [EPR22885-58] - BSA and Azide free (ab261914)
    Immunohistochemistry (Frozen sections) - Anti-AGXT antibody [EPR22885-58] - BSA and Azide free (ab261914)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen section of mouse liver tissue labeling AGXT with ab261910 at 1/100 dilution, followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution (green). The nuclear counter stain is DAPI (blue). Positive staining on mouse liver (PMID: 29883780).

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab261910).

  • Anti-AGXT antibody [EPR22885-58] - BSA and Azide free (ab261914)
    Anti-AGXT antibody [EPR22885-58] - BSA and Azide free (ab261914)

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