Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-AGXT antibody [EPR13232] - BSA and Azide free (ab250079)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13232] to AGXT - BSA and Azide free
  • Suitable for: WB, IP, IHC-P
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-AGXT antibody [EPR13232] - BSA and Azide free
    See all AGXT primary antibodies

  • Description

    Rabbit monoclonal [EPR13232] to AGXT - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IP, IHC-Pmore details
    Unsuitable for: Flow Cyt

  • Species reactivity

    Reacts with: Mouse, Human

  • Immunogen

    Recombinant fragment corresponding to Human AGXT.
    Database link: P21549

  • General notes

    ab250079 is the carrier-free version of ab178708 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab250079 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information. Mouse species is recommended by WB application. We have preliminary internal testing data to indicate this antibody may not react with mouse in IHC.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-AGXT antibody [EPR13232] - BSA and Azide free (ab250079)
    Western blot - Anti-AGXT antibody [EPR13232] - BSA and Azide free (ab250079)
    All lanes : Anti-AGXT antibody [EPR13232] (ab178708) at 1/1000 dilution

    Lane 1 : HepG2 cell lysate
    Lane 2 : Human fetal liver tissue lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP-conjugated at 1/2000 dilution

    Predicted band size: 43 kDa



    This data was developed using ab178708, the same antibody clone in a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AGXT antibody [EPR13232] - BSA and Azide free (ab250079)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AGXT antibody [EPR13232] - BSA and Azide free (ab250079)
    This data was developed using ab178708, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human hepatocellular carcinoma tissue labeling AGXT with ab178708 at 1/100 dilution. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AGXT antibody [EPR13232] - BSA and Azide free (ab250079)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AGXT antibody [EPR13232] - BSA and Azide free (ab250079)
    This data was developed using ab178708, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling AGXT with ab178708 at 1/100 dilution. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Immunoprecipitation - Anti-AGXT antibody [EPR13232] - BSA and Azide free (ab250079)
    Immunoprecipitation - Anti-AGXT antibody [EPR13232] - BSA and Azide free (ab250079)
    This data was developed using ab178708, the same antibody clone in a different buffer formulation.Western blot analysis on immunoprecipitation pellet from (1) Human fetal liver cell lysate or (2) 1X PBS (negative control) immunoprecipitated using ab170891 at 1/10 dilution. Detection utilized HRP-conjugated anti-rabbit IgG which preferentially detects the non-reduced form of rabbit IgG.
  • Western blot - Anti-AGXT antibody [EPR13232] - BSA and Azide free (ab250079)
    Western blot - Anti-AGXT antibody [EPR13232] - BSA and Azide free (ab250079)

    This data was developed using ab178708, the same antibody clone in a different buffer formulation.

    Western blot analysis of Mouse liver (20µg) labelling AGXT with ab178708 at 1/2000 in 5% NFDM/TBST. A Peroxidase conjugated Goat Anti-Rabbit IgG, (H+L), (ab97051) (1/20000) was used as the secondary antibody.

  • Anti-AGXT antibody [EPR13232] - BSA and Azide free (ab250079)
    Anti-AGXT antibody [EPR13232] - BSA and Azide free (ab250079)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-AGXT antibody [EPR13232] - BSA and Azide free (ab250079)

© 2021 Astana Biomed Group LLP. All rights reserved.