Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Quiescin Q6 antibody [EPR21866] (ab235444)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR21866] to Quiescin Q6
  • Suitable for: WB, Flow Cyt, IP, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-Quiescin Q6 antibody [EPR21866]
    See all Quiescin Q6 primary antibodies

  • Description

    Rabbit monoclonal [EPR21866] to Quiescin Q6

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: BxPC-3, MCF7, MDA-MB-231, PANC-1 and T-47D whole cell lysates; Human breast cancer lysate. IHC-P: Human breast ductal cancer and pancreatic cancer tissue. IP: MDA-MB-231 whole cell lysate. Flow Cyt: MDA-MB-231 cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR21866

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-Quiescin Q6 antibody [EPR21866] (ab235444)
    Western blot - Anti-Quiescin Q6 antibody [EPR21866] (ab235444)
    All lanes : Anti-Quiescin Q6 antibody [EPR21866] (ab235444) at 1/1000 dilution

    Lane 1 : BxPC-3 (human pancreas adenocarcinoma cell line) whole cell lysate at 20 µg
    Lane 2 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate at 20 µg
    Lane 3 : MDA-MB-231 (human breast adenocarcinoma cell line) whole cell lysate at 20 µg
    Lane 4 : PANC-1 (human pancreatic epithelial cancinoma cell line) whole cell lysate at 10 µg
    Lane 5 : T-47D (Human ductal breast epithelial tumor cell line) whole cell lysate at 10 µg
    Lane 6 : Human breast cancer lysate at 10 µg

    Secondary
    Lanes 1-5 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
    Lane 6 : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution

    Predicted band size: 83 kDa
    Observed band size: 67,83 kDa
    why is the actual band size different from the predicted?



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure times: Lanes 1-3 & 5: 3 minutes, Lane 4: 37 seconds, Lane 6: 103 seconds.

    Quiescin Q6 has two isoforms due to expression from a long (Quiescin Q6-L) and a short (Quiescin Q6-S) transcript.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Quiescin Q6 antibody [EPR21866] (ab235444)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Quiescin Q6 antibody [EPR21866] (ab235444)

    Immunohistochemical analysis of paraffin-embedded human pancreatic cancer tissue labeling Quiescin Q6 with ab235444 at 1/200 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Cytoplasmic staining in human pancreatic cancer (PMID: 19795908, 21989104) is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

    Heat-mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Quiescin Q6 antibody [EPR21866] (ab235444)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Quiescin Q6 antibody [EPR21866] (ab235444)

    Immunohistochemical analysis of paraffin-embedded human breast ductal cancer tissue labeling Quiescin Q6 with ab235444 at 1/200 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Cytoplasmic staining in human breast ductal cancer (PMID: 23536962) is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

    Heat-mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Flow Cytometry - Anti-Quiescin Q6 antibody [EPR21866] (ab235444)
    Flow Cytometry - Anti-Quiescin Q6 antibody [EPR21866] (ab235444)

    Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized MDA-MB-231 (Human breast adenocarcinoma cell line) cells labeling Quiescin Q6 with ab235444 at 1/60 (red) compared with a Rabbit monoclonal IgG (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077), at 1/2000 dilution was used as the secondary antibody.

  • Immunoprecipitation - Anti-Quiescin Q6 antibody [EPR21866] (ab235444)
    Immunoprecipitation - Anti-Quiescin Q6 antibody [EPR21866] (ab235444)

    Quiescin Q6 was immunoprecipitated from 0.35 mg of MDA-MB-231 (Human breast adenocarcinoma cell line) whole cell lysate with ab235444 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab235444 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.

    Lane 1: MDA-MB-231 whole cell lysate 10 μg (Input).
    Lane 2: ab235444 IP in MDA-MD-231 whole cell lysate.
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab235444 in MDA-MD-231 whole cell lysate.

    Blocking/Dilution buffer: 5% NFDM/TBST.
    Exposure time: 30 seconds.

    Quiescin Q6 has two isoforms due to expression from a long (Quiescin Q6-L) and a short (Quiescin Q6-S) transcript.

  • Anti-Quiescin Q6 antibody [EPR21866] (ab235444)
    Anti-Quiescin Q6 antibody [EPR21866] (ab235444)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-Quiescin Q6 antibody [EPR21866] (ab235444)

© 2021 Astana Biomed Group LLP. All rights reserved.